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Your search keyword '"Gargari M"' showing total 3 results
Start Over Author "Gargari M" Journal international journal of immunopathology and pharmacology
3 results on '"Gargari M"'

1. The use of a new chemical device based on silver and cationic surfactants as a new approach for daily oral hygiene: A preliminary study on a group of periodontal patients

Author

Ottria Liliana, D'Orto Ornella, Candotto Valentina, Lauritano Dorina, Bolzoni Alessandro, Palmieri Annalisa, Gargari Marco, Michele Di Girolamo, Lauritano, D, Palmieri, A, D'Orto, O, Bolzoni, A, Ottria, L, Gargari, M, Michele, D, and Candotto, V

Subjects
Adult, Male, Silver, Test group, Immunology, Color, Dentistry, Placebo, Oral hygiene, Settore MED/28, Surface-Active Agents, 03 medical and health sciences, chemistry.chemical_compound, Economica, 0302 clinical medicine, medicine, Humans, Immunology and Allergy, LS7_2, Molecular Biology in Dentistry, Original Research Article, bacterial load, chronic periodontitis, oral biofilm, red complex, chronic periodontiti, Pharmacology, business.industry, Chlorhexidine, bacterial load, oral biofilm, MED/28 - MALATTIE ODONTOSTOMATOLOGICHE, chronic periodontitis, 030206 dentistry, Oral Hygiene, Red complex, medicine.disease, Chronic periodontal disease, Chronic periodontitis, Anti-Bacterial Agents, Quaternary Ammonium Compounds, chemistry, red complex, Female, Periodontal Index, business, Student's t-test, Didecyldimethylammonium chloride, 030215 immunology
Abstract

The aim of this study was to evaluate the abatement power of oral microbial loading of a new gel formulation based on the complex silver-2-mercaptobenzoate, chlorhexidine digluconate and didecyldimethylammonium chloride (named ADC) through polymerase chain reaction (PCR). The study sample consists of a group of 20 patients with chronic periodontal disease. Patients were over 25 years of age and did not undergo surgical or non-surgical periodontal treatment in the previous 6 months. The study sample was allotted into two groups of 10 patients each, homogeneous by age and sex. The test group received a bottle containing ADC gel, while the control group received an identical one containing placebo, similar to ADC in consistence, colour, taste and odour. Sub-gingival samples of four sites, one in each quadrant, of greatest probing depth in each patient were used. Microbiological analyses were performed at baseline and at day 15. Paired t test was performed to detect statistical significant reduction in total bacterial loading and oral pathogens in the study groups. The analysis showed a statistically significant reduction in the total bacterial loading evaluated pre- and post-treatment ( P = 0.029) in the study groups. In the control group, the decrease in total bacterial loading was not significant ( P = 0.279). Clinically, ADC gel does not have any side effects and discomfort such as pain, burning, tingling sensation or numbness and produces no adverse reactions in time. Our study aimed to evaluate the efficacy of a new chemical formulation with antibacterial properties to use for daily oral hygiene with a preliminary study. Our results showed a statistically significant reduction in total bacterial loading after treatment, but the limitations of our study do not allow us to demonstrate the clinical efficacy of the ADC gel.

Published
2019
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2. Innovative approach for the in vitro research on biomedical scaffolds designed and customized with CAD-CAM technology.

Author

Marrelli M, Pujia A, Palmieri F, Gatto R, Falisi G, Gargari M, Caruso S, Apicella D, Rastelli C, Nardi GM, Paduano F, and Tatullo M

Subjects
Adult, Cell Proliferation drug effects, Cells, Cultured, Computer-Aided Design, Dental Pulp drug effects, Humans, Materials Testing methods, Palladium pharmacology, Stem Cells drug effects, Titanium pharmacology, Zirconium pharmacology, Biocompatible Materials pharmacology, Regenerative Medicine methods, Tissue Scaffolds chemistry
Abstract

Introduction: Studies on biomaterials involve assays aimed to assess the interactions between the biomaterial and the cells seeded on its surface. However, the morphology of biomaterials is heterogeneous and it could be tricky to standardize the results among different biomaterials and the classic plastic plates. In this light, we decided to create, by means of computer-aided design (CAD) technology, a standardized sample model, with equal shape and sizes, able to fit into a classic shape of a 96-wells tissue culture plate (TCP)., Methods: The design of this sample consists of a hole in the top in order to allow the injected cells to settle without them being able to slip from the sides of the sample to the bottom of the TCP wells. This CAD project is made using the software Pro-Engineer. The sample will totally fill the wells of the 96-well TCP. Dental pulp stem cells have been used to assess the ability of the different sample to support and promote the cell proliferation., Results: Twelve titanium, 12 gold-palladium, and 12 zirconium oxide customized samples were designed by means of the software cam powermill, by importing the .stl file created in Pro-Engineer software. The proliferation rate of the tested scaffolds showed to be similar to the control in the group with the customized shape., Conclusion: We think that our method can be useful to test different types of scaffolds when a greater accuracy of the measurements is desirable in order to verify the cell behavior of these scaffolds. Our innovative method can improve the standardization process in the evaluation of cell behavior on different biomaterials to open the way to more reliable tests on biomatrices functionalized with drugs or growth factors applied to the future regenerative medicine., (© The Author(s) 2016.)

Published
2016
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3. Mechanical influence of tissue culture plates and extracellular matrix on mesenchymal stem cell behavior: A topical review.

Author

Tatullo M, Marrelli M, Falisi G, Rastelli C, Palmieri F, Gargari M, Zavan B, Paduano F, and Benagiano V

Subjects
Humans, Tissue Culture Techniques, Tissue Engineering, Extracellular Matrix physiology, Mesenchymal Stem Cells physiology
Abstract

Tissue engineering applications need a continuous development of new biomaterials able to generate an ideal cell-extracellular matrix interaction. The stem cell fate is regulated by several factors, such as growth factors or transcription factors. The most recent literature has reported several publications able to demonstrate that environmental factors also contribute to the regulation of stem cell behavior, leading to the opinion that the environment plays the major role in the cell differentiation.The interaction between mesenchymal stem cells (MSCs) and extracellular environment has been widely described, and it has a crucial role in regulating the cell phenotype. In our laboratory (Tecnologica Research Institute, Crotone, Italy), we have recently studied how several physical factors influence the distribution and the morphology of MSCs isolated from dental pulp, and how they are able to regulate stem cell differentiation. Mechanical and geometrical factors are only a small part of the environmental factors able to influence stem cell behavior, however, this influence should be properly known: in fact, this assumption must be clearly considered during those studies involving MSCs; furthermore, these interactions should be considered as an important bias that involves an high number of studies on the MSCs, since in worldwide laboratories the scientists mostly use tissue culture plates for their experiments., (© The Author(s) 2015.)

Published
2016
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