The Canary Islands were initially colonized around 200 CE by North African Berber populations who brought with them domestic plants and animals. These communities remained isolated until the arrival of Europeans in the Late Middle Ages which triggered the conquest of the archipelago. Its geostrategic location in the framework of Atlantic expansion facilitated the arrival of people, knowledge, goods, plants, and animals which served to shape the new Canarian society. This study explores the dietary practices of this period through the zooarchaeological analysis of the faunal remains recovered at the Hospital de San Martín (fifteenth to the eighteenth centuries), Las Palmas (Gran Canaria, Spain). Our results suggest that caprines were the main taxa consumed. This follows both the pre-colonial Indigenous tradition and, according to historical sources, the subsequent European period. The faunal assemblage recovered also reveals the first directly radiocarbon-dated cases in the Canary Islands of three hitherto unknown species: cattle, chicken, and rabbit. Moreover, the results evidence a progressive increase throughout the Modern Era of chicken and rabbit, and that translocating these new species to the archipelago during the European expansion led to a diversification of meat resources. [ABSTRACT FROM AUTHOR]