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Background:   miR-29c-3p  manages a set of genes involved in regenerative medicine, and It seems that hyperglycemia in diabetic patients influences the power of stem cells to tissue regeneration the difficulties of diabetes by affecting the expression  miR-29c-3p  in mesenchymal stem cells. The study aims to analyze the effect of various glucose concentrations on the miR -29c-3p expression  in mesenchymal stem cells. Materials and Methods:  After receiving donated mesenchymal stem cells from Tarbiat Modares University, these cells were cultivated in a DMEM culture medium, including three different concentrations of glucose 250, 140, and 100 mg/dl. RNA was extracted from these cells after 72 hours, the Real-Time PCR technique assessed the expression of miR-29c-3p, and the results were analyzed by REST software. Results:   miR-29c-3p  expression in cells at concentrations of 140 and 250 mg/dL compared to typical situations (100 mg/dl) was significantly decreased (P˂0.05), which declined at a concentration of 250 mg/dl was more. Conclusion:  Reduced  miR-29c-3p  expression in mesenchymal stem cells in chronic and mild diabetic situations demonstrated that diabetes might be one of the significant reasons for mesenchymal stem cells' reduced ability to repair tissue damage., Competing Interests: The authors declare that they have no competing interests., (Copyright © 2024 Tehran University of Medical Sciences.)