The yield and chemical composition of the seed oil ofCelastrus paniculatuswere determined by solvent extraction and gas chromatography-mass spectrometry. The yield of crude fatty oil was found to be 45.5% in the mature seeds on dry weight basis. Fourteen compounds, constituting 97.57% of the oil, were identified. The oil was rich in unsaturated fatty acids (70.11%) followed by saturated fatty acids (25.2%). Oleic acid (54.42%), palmitic acid (20.0%), linoleic acid (15.51%), and stearic acid (4.18%) were the main fatty acids in the oil together with four other minor fatty acids. The current study identifies the novel compounds not previously reported inC. paniculatusoil, including 1,4-benzenediol (0.46%), cinnamic acid (0.15%), 2,6-di-tert-butyl-p-benzoquinone (0.03%), butylated hydroxytoluene (0.4%), and eudalene (0.16). [ABSTRACT FROM AUTHOR]