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Water and swab samples were collected from 13 hospitals and analyzed for Legionella counts. Legionella was detected in eight out of 13 hospitals and in 22 of 130 water and swab-collected samples. A total of 72.7% of the strains were L. pneumophila ser. 1, 22.7% were L. pneumophila ser. 2-14, and 4.5% did not belong to any of these groups. AFLP typing of the L. pneumophila ser. 1 strains generated two distinguishable AFLP types. There was no significant correlation to the sample type with Legionella recovery. Legionella isolation was more likely to occur in the cooling towers than the water system. Water temperatures of 30-40 degrees C seem to favor Legionella growth. Of the 265 serum samples taken from the medical and technical staff for the control of IgG titre, 89.4% were negative, 7.2% were positive, and for 3.4% the result was doubtful. No association between IgG titre and maximum observed level of Legionella occurrence was detected.

In this work a representative sample of 11 indoor and outdoor swimming pools were investigated with respect to critical physicochemical, health-related, microbiological and technical parameters. These pools were used either as competition (athletic) or as recreation pools. Useful correlations of the above parameters were deduced and properly attributed. Results were checked against well-established standards, such as DIN 19643, showing poor compliance with them in all parameters investigated. Thus, compliance for health-related parameters varied from 0 to 100%, for physical and chemical parameters from 20 to 100%, for microbiological parameters from 45 to 91% and for technical parameters from 27 to 36%. The most critical parameters implicated for poor water quality were Redox potential, turbidity and the appearance of certain opportunistic pathogens ( Staphylococcus aureus and Pseudomonas aeruginosa ). These were attributed to poor process design of pools and lack of sufficient vocational training of the ...

For the disposal of sewage, sludge landfill is currently the practice adopted in Greece. Thirteen wastewater treatment plants located throughout Greece (islands excluded) were investigated over a period of 1 year in order to determine sludge microbiological quality. The time of exposure of the samples to environmental conditions and stress varied considerably, from a few hours to several months. The mean values for total coliforms were 4 to > 2400/100 ml and for faecal coliforms 2400/100. Salmonellae were isolated from all plants with the exception of two. Parasites were isolated from five treatment plants. There was no difference in the bacterial indicator counts of faecal pollution or the presence of salmonellae and parasites according to the time of disposal of the sludge to the fields. The samples taken from the soil, or from flowers and vegetables which grow in the area of the sludge disposal were mostly negative for faecal pollution bacteria indicators. Salmonellae and parasites were not recovered from any samples. Five of the 16 serotypes reported from the National Salmonella Reference Center were present in the sludge samples.