1. Isolation of a sulphated polysaccharide from a recently discovered sponge species (Celtodoryx girardae) and determination of its anti-herpetic activity
- Author
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Nathalie Bourgougnon, Elodie Lahaye, Bertrand Perrin, Olivier Sire, Diane Defer, Zalilawati Mat Rashid, Philippe Douzenel, Laboratoire d'Ingénierie des Matériaux de Bretagne (LIMATB), Université de Bretagne Sud (UBS)-Institut Brestois du Numérique et des Mathématiques (IBNM), and Université de Brest (UBO)-Université de Brest (UBO)-Université de Brest (UBO)
- Subjects
Spectrophotometry, Infrared ,Drug Evaluation, Preclinical ,Herpesvirus 1, Human ,medicine.disease_cause ,Antiviral Agents ,Biochemistry ,Virus ,Microbiology ,03 medical and health sciences ,Polysaccharides ,Structural Biology ,Chlorocebus aethiops ,medicine ,Animals ,[SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering ,Least-Squares Analysis ,Cytotoxicity ,Vero Cells ,Molecular Biology ,ComputingMilieux_MISCELLANEOUS ,030304 developmental biology ,chemistry.chemical_classification ,Principal Component Analysis ,0303 health sciences ,biology ,030306 microbiology ,General Medicine ,Isolation (microbiology) ,biology.organism_classification ,In vitro ,Porifera ,3. Good health ,Sponge ,Herpes simplex virus ,chemistry ,Chromatography, Gel ,Vero cell ,Glycoprotein - Abstract
Exopolysaccharides (EPS) were extracted from a sponge, Celtodoryx girardae recently discovered in the Golfe du Morbihan in 2000. Sponge samples were collected monthly from November 2007 to May 2008. SEC analysis of EPS samples showed that they exhibit a unique molecular weight of approximately 800 kDa. However, infrared analysis revealed that structural seasonal variations occur. EPS fractions also exhibit significant sulphate contents and were screened in vitro for a potential antiviral activity against Herpes simplex virus type 1 (HSV-1). The best result was obtained with a sample collected in January which exhibits an EC(50) of 5.9 microg/mL without cytotoxicity on the Vero cell line. Experiments carried out to elucidate the mechanism of the EPS showed that the sulphated groups of EPS interact with the glycoproteins on the surface of the virus' membrane.
- Published
- 2009
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