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Active packaging has been recognized as an effective approach to extend the shelf life of food, but the rapid release of active substances limits the preservation effect. In this study, gallic acid (GA)-loaded ovalbumin (OVA)/chitosan (CS) nanoparticles with slow-release properties were prepared and embedded into the pectin matrix to refine the rapid release of GA in the pectin and elongate the shelf life of salmon fillets. Our results showed that GA could be released continuously from the OVA/CS nanoparticles. The pectin film incorporated with GA-loaded OVA/CS nanoparticles exhibited good light barrier and mechanical properties. The opacity value of the film reached 1.65 ± 0.06 UA/mm, and the tensile strength and elongation at break were 15.97 ± 1.55 MPa and 7.29 ± 0.42 %, respectively. In addition, the pectin film combined with GA-loaded OVA/CS nanoparticles showed improved antibacterial activity against two common biogenic amine-producing bacteria (Morganella morganii and Escherichia coli). Moreover, the nanocomposite film delayed salmon fillets' biogenic amine generation, and the shelf life was extended by 3 days compared with the control group. These promising properties supported using the GA-loaded OVA/CS nanoparticle-pectin films as preservation materials for fish. • Gallic acid-loaded ovalbumin/chitosan nanoparticles with sustained-release effect. • Nanocomposition film consisted of pectin and the nanoparticles was prepared. • The nanocomposition film endowed the salmon fillets with extended shelf-life. • The film delayed the growth of biogenic amines and amine-producing bacteria. [ABSTRACT FROM AUTHOR]

Pectin and starch crystals were modified by ethyl gallate and octadecyl-trimethoxysilane, respectively, followed by using acylated pectin (AP) and alkylated starch crystals (ASCs) as bioactive reagents and hydrophobic enhancers to improve the physiochemical properties of gelatin-based films and evaluate their coating preservation effects on golden pomfret. The properties of AP and ASC were investigated by Fourier transform infrared spectroscopy (FTIR), ultraviolet–visible spectroscopy (UV–vis), proton-nuclear magnetic resonance (1H NMR) and X-ray diffraction (XRD). The ethyl-gallate-modified pectin/gelatin (AP/G) containing 3 % ASC (AP/G/ASC-3 %) was shown to have the maximum tensile strength and Young's modulus of all the tested composite films. The AP/G containing 10 % ASC exhibited a water contact angle higher than 94°, coupled with a significant improvement in UV-shielding efficiency. FTIR and SEM analysis of the AP/G/ASC-3 % film indicated that the molecular interactions in the composite film components were noncovalent linkages, including hydrogen bonds, hydrophobic interactions, and electrostatic interactions, contributing to homogeneous and smooth microstructures. Additionally, the solutions of AP/G and AP/G/ASC composite films presented obvious antioxidant and antibacterial activities against Escherichia coli and Staphylococcus aureus. Furthermore, the AP/G and AP/G/ASC active coatings could effectively inhibit lipid oxidation and improve the textural acceptability of golden pomfret (Trachinotus blochii) fillets during 4 °C storage. • Alkylated starch crystal (ASC) and acylated pectin (AP) were synthesized as enhancers. • ASC and AP enhanced comprehensive properties of gelatin (G)-based composite film. • Gold pomfret shelf life was effectively prolonged via ASC/AP/G coating. [ABSTRACT FROM AUTHOR]