1. Extracellular vesicles secreted by Schistosoma mansoni contain protein vaccine candidates.
- Author
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Sotillo, Javier, Pearson, Mark, Potriquet, Jeremy, Becker, Luke, Pickering, Darren, Mulvenna, Jason, and Loukas, Alex
- Subjects
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VESICLES (Cytology) , *SCHISTOSOMA mansoni , *TRANSMISSION electron microscopy , *PROTEOMICS , *PROTOZOAN virulence factors , *VACCINE effectiveness - Abstract
Herein we show for the first time that Schistosoma mansoni adult worms secrete exosome-like extracellular vesicles ranging from 50 to 130 nm in size. Extracellular vesicles were collected from the excretory/secretory products of cultured adult flukes and purified by Optiprep density gradient, resulting in highly pure extracellular vesicle preparations as confirmed by transmission electron microscopy and Nanosight tracking analysis. Extracellular vesicle proteomic analysis showed numerous known vaccine candidates, potential virulence factors and molecules implicated in feeding. These findings provide new avenues for the exploration of host–schistosome interactions and offer a potential mechanism by which some vaccine antigens exert their protective efficacy. [ABSTRACT FROM AUTHOR]
- Published
- 2016
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