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Hypothetical proteins (HPs) are those whose sequence is present but nothing is known about their structural and functional annotations. Elucidation of structural and functional insights would be effective enough in perceiving the protein interactions and their involvement in various pathways and networks. Ananas comosus is considered as third most essential tropical fruit and is given more consideration from the commercial point of view; therefore, in this research, it is taken for analysis using different approaches of bioinformatics. On the basis of subcellular localization and secondary structure analysis, it was suggested that HP is the nuclear protein consisting of α helices and more abundant coils. Homology modeling has been done through Swiss Model to determine their template structure but template identity score is not enough which uncover the fact that the protein OAY63476.1 is unpredicted in-vitro. In order to predict the 3D structure of protein, Phyre 2 server is utilized. Results are validated by different strategies revealing the stability of the developed model. The quality factor of this protein is 45.28. Functional analysis and domain identification have been performed by using NCBI-CDD and Pfam which suggested that protein contains ring variant domain. Comparative genome analysis has been performed with other proteins of plants which demonstrate that the selected protein has the highest similarity. This study paves the way in order to determine the structural and functional annotations of other uncharacterized proteins and effective in exploring other novel proteins along with their functions in the same way as that of this research study.

Osteoporosis occurs because of the Calcitonin-Related Polypeptide Alpha (CALCA) gene. At present, Caltrate600, Boniva and Alendronate are viewed as dynamic drugs to cure osteoporosis. Chronic lymphocytic leukemia occurs because of the ABL1 proto oncogene. Currently, Rituximab, Fludarabine and Cytarabine are utilized as monoclonal antibodies against this ailment. Drug repositioning is a new rising field of reusing previous drugs, safeguarding retired drugs and developing licenses to make lives easy. The main objective of this research was repositioning of Alendronate and Cytarabine in order to use them in other diseases, too. Interactions of each of these drugs with many off-target proteins were identified. Alendronate presented strong interactions with FPPS, Hydroxylapatite, PTPRS, PTPRE, PTN4 and GGPPS. Cytarabine demonstrated strong interactions with DNA and DPOLB. After screening a great number of drugs which are accustomed to cure mutations of those off-target genes and proteins, their ill effects were compared, and it is suggested that Alendronate and Cytarabine have less side effects than different other drugs utilized for the same interacting targets. Both Alendronate and Cytarabine can be repositioned to cure well known carcinomas and different diseases.

Hypothetical proteins (HPs) are the proteins whose presence has been anticipated, yet in vivo function has not been built up. Illustrating the structural and functional privileged insights of these HPs might likewise prompt a superior comprehension of the protein-protein associations or networks in diverse types of life. Bananas (Musa acuminata spp.), including sweet and cooking types, are giant perennial monocotyledonous herbs of the order Zingiberales, a sister grouped to the all-around considered Poales, which incorporate oats. Bananas are crucial for nourishment security in numerous tropical and subtropical nations and the most prominent organic product in industrialized nations. In the present study, the hypothetical protein of M. acuminata (Banana) was chosen for analysis and modeling by distinctive bioinformatics apparatuses and databases. As indicated by primary and secondary structure analysis, XP_009393594.1 is a stable hydrophobic protein containing a noteworthy extent of α-helices; Homology modeling was done utilizing SWISS-MODEL server where the templates identity with XP_009393594.1 protein was less which demonstrated novelty of our protein. Ab initio strategy was conducted to produce its 3D structure. A few evaluations of quality assessment and validation parameters determined the generated protein model as stable with genuinely great quality. Functional analysis was completed by ProtFun 2.2, and KEGG (KAAS), recommended that the hypothetical protein is a transcription factor with cytoplasmic domain as zinc finger. The protein was observed to be vital for translation process, involved in metabolism, signaling and cellular processes, genetic information processing and Zinc ion binding. It is suggested that further test approval would help to anticipate the structures and functions of other uncharacterized proteins of different plants and living being.