1. Effect of 5-aminolevulinic acid on gene expression and presence of NKG2D receptor on NK cells.
- Author
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Dashti Gerdabi N, Ghafourian M, Nakajima M, Iranparast S, and Khodadadi A
- Subjects
- Adult, Cell Differentiation drug effects, Cell Differentiation immunology, Cells, Cultured, Drug Screening Assays, Antitumor, Gene Expression Regulation immunology, Healthy Volunteers, Humans, Interferon-gamma metabolism, Killer Cells, Natural immunology, Killer Cells, Natural metabolism, Lymphocyte Activation drug effects, NK Cell Lectin-Like Receptor Subfamily K metabolism, Neoplasms drug therapy, Neoplasms immunology, Primary Cell Culture, Aminolevulinic Acid pharmacology, Killer Cells, Natural drug effects, NK Cell Lectin-Like Receptor Subfamily K agonists
- Abstract
Natural killer (NK) cells are involved in innate and acquired immunity, stimulating and enhancing immune responses via secretion of IFN-γ and TNF-α. NKG2D is among the most important NK's stimulant receptors, the ligands of which are elevated on cancerous and virus-infected cells. We analyzed effect of 5-ALA on gene expression and receptor presentation of NKG2D, which is present on peripheral blood NK cells. Mononuclear cells were isolated from the venous blood samples of healthy individuals. RNA extraction and cDNA synthesis were performed after exposure of samples to 5-ALA, and gene expression was evaluated using Real-Time PCR, and the receptor presence rate on the cell surface was evaluated by flow-cytometry analysis. The results showed the gene expression of NKG2D and the presence of its receptor on NK cells were increased.5-ALA can be used to activate NK cells in their killing activity, preventing the growth and metastasis of cancerous cells., (Copyright © 2021 Elsevier B.V. All rights reserved.)
- Published
- 2021
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