1. DA-9601 suppresses 2, 4-dinitrochlorobenzene and dust mite extract-induced atopic dermatitis-like skin lesions
- Author
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Choi, Eun-Ju, Lee, Soyoung, Hwang, Ji-Sun, Im, Sin-Hyeog, Jun, Chang-Duk, Lee, Hyun-Shik, and Kim, Sang-Hyun
- Subjects
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SKIN inflammation , *HOUSE dust mites , *DINITROCHLOROBENZENE , *ANTI-inflammatory agents , *HISTOPATHOLOGY , *HISTAMINE , *TUMOR necrosis factors , *THERAPEUTICS - Abstract
Abstract: DA-9601 (Stillen™) is a novel anti-peptic formulation prepared from the ethanol extracts of Artemisia asiatica possessing anti-oxidative, anti-allergic and anti-inflammatory activities. However, their effect on atopic dermatitis (AD) has not been studied yet. In this study, we report that topical application of DA-9601 suppressed house dust mite extract (Dermatophagoides farinae extract, DFE) and 2, 4-dinitrochlorobenzene (DNCB)-induced AD-like skin lesions in BALB/c mice model. We established atopic dermatitis model in BALB/c mice by repeated local exposure of DFE/DNCB to the ears. Repeated alternative treatment of DFE/DNCB caused AD-like lesions. DA-9601 reduced AD-like skin lesions based on ear thickness and histopathological analysis, and serum IgE levels. DA-9601 inhibited mast cell infiltration into the ear and elevation of serum histamine in AD model. In addition, DA-9601 suppressed DFE/DNCB-induced expression of IL-4, IL-13, IL-31, and TNF-α in the ears. Taken together, our results showed that topical application of DA-9601 exerts beneficial effects in animal model of AD, suggesting that DA-9601 might be a candidate for the treatment of AD. [Copyright &y& Elsevier]
- Published
- 2011
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