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Aim The aim of this study was to examine the apical sealing ability of five root canal sealers using a fluid transport model. Methodology A comparison of root canal sealers AH26, AH Plus, Diaket, Apexit, and Ketac-Endo were tested on 60 single-rooted teeth. The coronal part of each tooth was removed at the amelo-cemento junction, and also 3 mm of the root tip. Root canals were instrumented using the ‘step-back’ technique with Gates Glidden drills and irrigated with 2.5% NaOCl. The specimens were divided into five groups of 10 samples each and filled with test materials and gutta-percha points by the cold lateral condensation technique. Ten teeth were used as a control group, out of which five served as negative and five as positive controls. The leakage was measured by the movement of an air bubble in a capillary glass tube connected to the experimental root section. Results The differences in leakage amongst Ketac-Endo (0.318μL; SD 0.084), AH26 (0.319μL; SD 0.075), AH Plus (0.330L; SD 0.085) Apexit (0.360μL; SD 0.127) and Diaket (0.387μL; SD 0.140) were not statistically significant (P 0.05). Conclusions Under the conditions of this study, all five sealers produced a satisfactory seal. [ABSTRACT FROM AUTHOR]