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The aim of this study was to evaluate the applicability of a carbon dioxide (CO 2 ) laser as a means of achieving early closure of gingival flaps after periodontal surgery. In this study, an incision was made from the maxillary premolar to the canine tooth in dogs. Full thickness flaps were raised, and mucoperiosteal flaps were created. After repositioning the mucoperiosteal flaps in the experiment group, CO 2 lasers were applied to the incision sites, and the flaps were closed with the laser. In the control group, they were closed with simple silk. Animals were sacrificed at postoperative days 7 and 14, and histological examinations were performed. The results showed that although epithelial invasion was observed along the incision areas 7 days after treatment in the control groups, complete closure of the incision area and regular arrangement of fibers in the connective tissue beneath the epithelium were observed in the group treated with lasers. These results suggest that closure of gingival flaps with a CO 2 laser may reduce the possibility of infection at initial incision sites.