1. Global DNA hypomethylation has no impact on lung function or serum inflammatory and fibrosis cytokines in asbestos-exposed population.
- Author
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Yu, Min, Lou, Jianlin, Xia, Hailing, Zhang, Min, Zhang, Yixiao, Chen, Junqiang, Zhang, Xing, Ying, Shibo, Zhu, Lijin, Liu, Lihong, and Jia, Guang
- Subjects
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ASBESTOS & health , *LUNG diseases , *BIOLOGICAL tags , *DNA methylation , *ASBESTOS , *FIBROSIS , *CYTOKINES ,RISK factors - Abstract
Purpose: To examine the effect of asbestos exposure on global DNA methylation and determine whether lung function and inflammatory and fibrosis biomarkers are correlated with the methylation state. Methods: A total of 26 healthy subjects without asbestos exposure (Group 1), 47 healthy subjects with exposure (Group 2), and 52 subjects with benign asbestos-related disorders (ARDs) (Group 3) participated in this cross-sectional study. Blood global 5-methylcytosine (5mC) and serum TNF-α, collagen IV, CCL5 and CC16 concentrations were analyzed using enzyme-linked immunosorbent assay-like assays. Spirometric maneuvers were performed to assess lung function. Results: Decreased 5mC levels were observed in Groups 2 and 3 compared to Group 1, irrespective of lung function ( p < 0.01). There was no significant change in 5mC between Groups 2 and 3. Overall, 5mC was negatively correlated with CCL5 and collagen IV ( p < 0.05), but no significant inverse relationship was found between 5mC and CCL5 or collagen IV in each group. Additionally, both 5mC and CC16 were inversely associated with FEV1/FVC% ( p = 0.001, adjusted R = 0.145) for non-smokers, and consistently significant inverse relationships were found between CC16 and FEV1/FVC%, independent of asbestos exposure. Conclusions: Asbestos exposure causes global DNA hypomethylation. DNA hypomethylation has no influence on serum biomarkers and lung function in asbestos-exposed population with or without pleural and pulmonary parenchymal abnormalities. [ABSTRACT FROM AUTHOR]
- Published
- 2017
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