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20 results on '"Ra, Chisei"'

8. IgE- and FcεRI-Mediated Enhancement of Surface CD69 Expression in Basophils: Role of Low-Level Stimulation

Author

Suzukawa, Maho, primary, Komiya, Akiko, additional, Yoshimura-Uchiyama, Chitose, additional, Kawakami, Ayako, additional, Koketsu, Rikiya, additional, Nagase, Hiroyuki, additional, Iikura, Motoyasu, additional, Yamada, Hirokazu, additional, Ra, Chisei, additional, Ohta, Ken, additional, Yamamoto, Kazuhiko, additional, and Yamaguchi, Masao, additional

Published
2007
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9. Induction of Basophil Desensitization in Physiological Medium: Enhancement after IgE-Dependent Upregulation of Surface IgE Binding on Basophils

Author

Komiya, Akiko, primary, Hirai, Koichi, additional, Iikura, Motoyasu, additional, Nagase, Hiroyuki, additional, Yamada, Hirokazu, additional, Miyamasu, Misato, additional, Ohta, Ken, additional, Morita, Yutaka, additional, Ra, Chisei, additional, Yamamoto, Kazuhiko, additional, and Yamaguchi, Masao, additional

Published
2003
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14. NC/Nga Mice: A Mouse Model for Atopic Dermatitis

Author

Suto, Hajime, primary, Matsuda, Hiroshi, additional, Mitsuishi, Koichi, additional, Hira, Kayako, additional, Uchida, Takafumi, additional, Unno, Tetsushi, additional, Ogawa, Hideoki, additional, and Ra, Chisei, additional

Published
1999
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19. Significantly high levels of anti-dsDNA immunoglobulin E in sera and the ability of dsDNA to induce the degranulation of basophils from chronic urticaria patients.

Author

Hatada Y, Kashiwakura J, Hayama K, Fujisawa D, Sasaki-Sakamoto T, Terui T, Ra C, and Okayama Y

Subjects
Adult, Chronic Disease, Dermatitis, Atopic immunology, Female, Humans, Immunoglobulin E blood, Immunoglobulin G blood, Immunoglobulin G immunology, Male, Middle Aged, Basophils immunology, Cell Degranulation immunology, DNA immunology, Immunoglobulin E immunology, Urticaria immunology
Abstract

Background: Chronic urticaria (CU) appears to be of autoimmune origin in about half of all patients, since several autoreactive immunoglobulin Gs (IgGs), such as anti-FcεRIα and anti-IgE, are detected in the sera of such patients. However, whether autoreactive IgE is associated with CU remains unclear. In this study, we attempted to identify autoreactive IgE antibodies in sera from patients with CU., Methods: Sera were collected from 67 normal subjects, 85 patients with CU and 28 patients with atopic dermatitis (AD). An autologous serum skin test (ASST) was performed on 27 of the CU patients. Autoreactive IgE and IgG levels against self-antigens were measured using enzyme-linked immunosorbent assays. The basophils were activated with dsDNA, and the CD63 expression level was examined using a fluorescence-activated cell sorter., Results: The anti-dsDNA IgE levels were significantly higher in patients with CU and AD than in normal subjects, but no differences in the anti-dsDNA IgG levels were seen. The levels of thioredoxin-, peroxiredoxin- and thyroglobulin-reactive IgE and IgG were not significantly higher in the CU patients than in the other 2 groups. There was no significant difference in the levels of anti-dsDNA IgE between ASST-positive and ASST-negative patients. The basophils from 2 out of 9 CU patients exhibited degranulation in response to dsDNA., Conclusions: Our data suggest that anti-dsDNA IgE is involved in the pathogenesis of some cases of CU., (Copyright © 2013 S. Karger AG, Basel.)

Published
2013
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20. Prolonged culture of mast cells with high-glucose medium enhances the Fc epsilon RI-mediated degranulation response and leukotriene C4 production.

Author

Kitahata Y, Nunomura S, Terui T, and Ra C

Subjects
Adenosine Triphosphate metabolism, Animals, Arachidonate 5-Lipoxygenase genetics, Calcimycin pharmacology, Calcium Signaling drug effects, Cell Survival drug effects, Cyclooxygenase 1 genetics, Cyclooxygenase 2 genetics, Gene Expression genetics, Immunoglobulin E immunology, Immunoglobulin E pharmacology, Interleukin-6 metabolism, Mast Cells drug effects, Mice, Mice, Inbred C57BL, Phospholipases A2, Cytosolic metabolism, Phosphorylation drug effects, Serine metabolism, Serum Albumin, Bovine immunology, Serum Albumin, Bovine pharmacology, Time Factors, beta-N-Acetylhexosaminidases metabolism, Cell Degranulation drug effects, Glucose pharmacology, Leukotriene C4 metabolism, Mast Cells physiology, Receptors, IgE metabolism
Abstract

Background: Mast cell-released chemical mediators such as histamine, leukotriene (LT) C(4) and prostaglandin (PG) D(2) lead to the onset of allergic disorders. ATP provided from glycolysis is essential for histamine release and LTC(4) secretion from mast cells upon Fc epsilon RI cross-linking, indicating that glucose is a primary environmental factor for mast cell activation. In this study, we investigated whether increases in concentrations of glucose in culture media affect the activation of bone marrow-derived mouse mast cells (BMMCs) upon Fc epsilon RI cross-linking., Methods: BMMCs were cultured in RPMI-1640 supplemented with varying concentrations (5.5, 11, 16.5, 22, 27.5 and 33 mM) of D-glucose for 3 h, or 1, 3 or 7 days. D-Mannitol was added to the medium containing 5.5 mMD-glucose for osmotic control. After culturing, these cells were sensitized with anti-TNP IgE and then stimulated with TNP-BSA., Results: We found that long-term culture (7 days) of BMMCs with 33 mMD-glucose increases the Fc epsilon RI-dependent release of beta-hexosaminidase and LTC(4) without affecting surface expression levels of Fc epsilon RI, intracellular ATP levels or calcium signaling. Biochemical analyses demonstrated that Fc epsilon RI-dependent phosphorylation of cytosolic phospholipase A(2) (cPLA(2)) at the Ser505 residue was significantly increased by culturing with 33 mM glucose., Conclusions: Taken together, our data suggest that glucose can augment Fc epsilon RI-mediated mast cell activation, particularly the degranulation response and LTC(4) secretion after prolonged culture of mast cells with high-glucose medium. Moreover, it is suggested that increased phosphorylation of cPLA(2) at the Ser505 residue contributes to the enhancement of LTC(4) secretion., ((c) 2010 S. Karger AG, Basel.)

Published
2010
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