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CuSe2, a promising raw material for absorption layer of CuInSe2-based solar cell, has outstanding electronic and magnetic properties. Here, almost pure pyrite CuSe2 particles were successfully synthesized by hot injection method using glucose as a substitute for toxic adjuvant. Through XRD, SEM and TEM, samples are carried out microscopic analysis for studying the change of microscopic structure and composition of products obtained from different experiments. The results showed that glucose plays a driving role in the synthesis of CuSe2 and pure pyrite CuSe2 can be obtained only when the temperature is suitable. [ABSTRACT FROM AUTHOR]