In this paper, a noninvasive portable prototype is presented for biomedical audio signal processing. The proposed prototype is suitable for monitoring the health of patients. The proposed hardware setup consists of a cost-effective microphone, multipurpose microcontroller and computing node that could be a mobile phone or general-purpose computer. Using parallel and high-performance techniques, this setup allows one to register and wirelessly multicast the recorded biomedical signals to computing nodes in real time. The developed prototype was used as a case study to estimate the heart rate (HR) from the captured biomedical audio signal. In this regard, the developed algorithm for estimating HR comprises three stages: preprocessing, separation, and HR estimation. In the first stage, the signal captured by the microphone is adapted for processing. Subsequently, a separation stage was proposed to alleviate the acoustic interference between the lungs and heart. The separation is performed by combining a non-negative matrix factorization algorithm, clustering approach, and soft-filter strategy. Finally, HR estimation was obtained using a novel and efficient method based on the autocorrelation function. The developed prototype could be used not only for the estimation of the HR, but also for the retrieval of other biomedical information related to the recording of cardiac or respiratory audio signals. The proposed method was evaluated using well-known datasets and compared with state-of-the-art algorithms for source-separation. The results showed that it is possible to obtain an accurate separation and reliable real-time estimation in terms of source separation metrics and relative error in the tested scenarios by combining multi-core architectures with parallel and high-performance techniques. Finally, the proposed prototype was validated in a real-world scenario. [ABSTRACT FROM AUTHOR]