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Simple Summary: The insect epidermis covers multiple parts of the insect body, fulfilling both structural and protective roles. Primarily composed of chitin and epidermal proteins, the insect epidermis displays considerable diversity among insects. The CPR family, the largest and most diverse group of cuticular protein (CP) genes identified to date, plays crucial roles in insect development, including body length regulation and cuticle formation. Steroid hormones, such as ecdysteroids, regulate the expression of epidermal proteins. In addition, the insecticide Methoxyfenozide mimics the action of 20E, thereby affecting insect growth. Our study on Heortia vitessoides Moore elucidates the role of HvCP3L and offers novel insights into pest management strategies for Aquilaria sinensis (Lour.) Spreng. Cuticle proteins (CPs) constitute a multifunctional family; however, the physiological role of Cuticle Protein 3-like (CP3L) in Heortia vitessoides Moore remains largely unclear. In this study, we cloned the HvCP3L gene from the transcriptional library of Heortia vitessoides Moore. RT-qPCR results revealed that HvCP3L exhibited high expression levels during the larval stage of Heortia vitessoides Moore, particularly at the L5D1 stage, observed in both larval and adult heads. Through RNA interference, we successfully silenced the HvCP3L gene, resulting in a significant reduction in the survival rate of Heortia vitessoides Moore, with the survival rate from larvae to adults plummeting to a mere 17.7%, accompanied by phenotypic abnormalities. Additionally, we observed that the knockdown of HvCP3L led to the inhibition of genes in the chitin pathway. Following exposure to methoxyfenozide stress, the HvCP3L gene exhibited significant overexpression, coinciding with phenotypic abnormalities. These findings underscore the pivotal role of HvCP3L in the growth and development of Heortia vitessoides Moore. [ABSTRACT FROM AUTHOR]

Simple Summary: The present study investigated the effects of different temperatures (15, 20, 25, 30, and 35 °C) on the growth and development of Habrobracon hebetor Say (Hymenoptera: Braconidae) on Ephestia elutella (Hübner) (Lepidoptera: Pyralidae) larvae. Habrobracon hebetor could complete growth and development when E. elutella larvae were used as hosts, and the developmental duration decreased with increasing temperature at 15, 20, 25, 30, and 35 °C. Taking into account factors such as the total number of eggs laid, egg hatchability, the number of emerged adults, emergence ratio, percentage of female offspring, innate rate of increase, and net reproductive rate, the optimal temperature for rearing of H. hebetor is 30 °C. Augmentative release of parasitoids has been an important component of integrated insect management for stored product protection. Understanding the effect of different temperatures on the growth and development of parasitoids is in favor of mass rearing of parasitoids. Habrobracon hebetor Say (Hymenoptera: Braconidae) is a highly cosmopolitan, gregarious ecto-parasitoid of a variety of Lepidopterous larvae. Thus, the growth and development of H. hebetor reared on Ephestia elutella (Hübner) (Lepidoptera: Pyralidae) larvae were investigated at 15, 20, 25, 30, and 35 °C. Habrobracon hebetor could complete growth and development, and the developmental duration decreased with increasing temperature at 15, 20, 25, 30, and 35 °C. The development threshold temperatures of H. hebetor eggs, larvae, pupae, and egg-to-adult stages were 13.89, 6.39, 9.24, and 9.29 °C, and the effective accumulated temperatures were 23.33, 46.40, 142.68, and 240.31 °C·d, respectively. The total number of eggs laid by H. hebetor, the hatching rate of H. hebetor eggs, and the percentage of female offspring reached the maximum of 192.39, 83.89%, and 74.04% at 30 °C, respectively. There was no significant difference in pupal survival rate in the temperature range of 15 °C to 35 °C. At 30 °C, the pre-oviposition duration of H. hebetor was the shortest (0.87 d). Therefore, the optimal rearing temperature of H. hebetor was 30 °C. The present results are useful for the large-scale rearing of H. hebetor using E. elutella larvae as hosts and effectively implementing the biological control of stored-product insects. [ABSTRACT FROM AUTHOR]