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Simple Summary: Female frog-biting midges exclusively feed on blood from frogs. They are attracted by the calling of male frogs and search for specific feeding sites on their host's body. To feed, these blood-feeding midges use a very short proboscis that is composed of six piercing structures and an enclosing component. We analyzed the morphology of the mouthparts using SEM and compared these with the well-studied proboscises of other blood-feeding flies. Females of Corethrella share more similarities to the very small blood-feeding short-proboscid biting midges, black flies and sand flies than to their more closely related long-proboscid mosquitoes. We interpret our findings in the functional context of a very short piercing proboscis and its possible specialization to pierce frogs. Females of frog-biting midges (Corethrellidae) obtain their blood meals from male calling frogs. While the morphology of the feeding apparatus is well studied in hematophagous Diptera that impact humans, frog-biting midges have received far less attention. We provide a detailed micromorphological examination of the piercing blood-sucking proboscis and maxillary palpus in three Corethrella species using scanning electron microscopy and histological semi-thin sectioning. We also compare the sensilla found on the proboscis tip and the palpus of Corethrella with other piercing blood-sucking Diptera. Corethrella spp. have a proboscis length of about 135 µm, equipped with delicate mandibular piercing structures composing the food canal together with the labrum and hypopharynx. Their proboscis composition is plesiomorphic and more similar to other short-proboscid hematophagous Culicomorpha (e.g., Simuliidae), in contrast to the phylogenetically more closely related long-proboscid Culicidae. As in other short-proboscid taxa, the salivary canal in Corethrella spp. transitions into an open salivary groove with one mandible forming a seal, whereas in Culicidae the salivary canal is closed until the tip of the proboscis. We discuss the possible functional constraints of very short, piercing blood-sucking proboscises (e.g., dimensions of host blood cells) that may limit the size of the food canal. [ABSTRACT FROM AUTHOR]

Simple Summary: Adult Yellowjackets only take up sugar-rich liquid for their daily diet. As these fluids are usually collected from sources with high amounts of various particles, such as fruit flesh or shards of bark, it is important to filter these particles, especially as these animals have a wasp waist with a constriction of the gut, through which the ingested fluid has to pass. We conducted experiments with variously-sized glass particles that were provided in a sugar solution. Female workers of the German Wasp were X-rayed after the food uptake. Possible areas of filtration were investigated with scanning electron microscopy to visualize the surface structures. We could identify two possible areas with filtration function. The first is at the frontal part of the mouthparts, at which the animals could prevent the uptake of particles that were larger than 0.2 mm. A second area inside the head with rows of interlinking hair-like structures enables much finer filtration. Particles that were bigger than 0.15 mm were reliably filtered. The particles that were filtered at this second filtration area were stored in a pouch-like organ inside the head. Smaller particles were also filtered, but some of these were able to pass. These results demonstrate fluid filtration, a poorly-studied topic of insect morphology and behavior. The mouthparts of Vespidae have evolved to forage various solid and liquid foods, such as animal prey, carbohydrate-rich fluids, as well as woody fibres for nest construction. Before nutritional fluids are ingested into the crop, bigger particles need to be filtered out. This study examined the functional morphology of the mouthparts, the preoral cavity, and the proximal alimentary tract inside the head focusing on this filtration process. The feeding organs and preoral cavity were studied using µCT and SEM that were complimented by feeding experiments with glass beads in workers of Vespula germanica. To visualize fluid ingestion into the head and alimentary tract, barium sulfate solution was used as contrast agent; a method that is rarely applied in entomology. Experimental results indicate that large glass beads (>212 µm) were filtered by the mouthpart structures before entering the preoral cavity. Smaller glass beads (152–212 µm) were found inside the infrabuccal pocket in front of the mouth. Morphological evidence indicates that cuticle structures of the epipharynx, hypopharynx, and cibarium filter this particle size inside the preoral cavity while glass beads < 152 µm reach the crop. A double fluid filtration system is proposed that is formed by (1) bristles of the mouthparts and (2) microtrichia of the preoral cavity. [ABSTRACT FROM AUTHOR]

Simple Summary: Flies with a particularly long proboscis are characteristic of flower-visiting insects in the Greater Cape Floristic Region of South Africa. We studied an endemic nemestrinid fly species in a small isolated area of semi-natural vegetation where these insects were the only flower visitors that could drink nectar from the available long-tubed flowers of one plant species. We examined the mouthpart structures that are important for nectar uptake and the length and diameter of the proboscis in comparison with the flower sizes. This local one-to-one interaction between the fly population and its nectar host flower gave the opportunity to quantify the nectar resources available for the nemestrinid flies at the study site. By comparing the offered nectar volumes before and after flower visits, the average meal size could be estimated. Assessments of the nectar levels from measured quantities and flower size allowed us to make predictions of how various proboscis lengths could reach nectar inside floral tubes. Several Prosoeca (Nemestinidae) species use a greatly elongated proboscis to drink nectar from long-tubed flowers. We studied morphological adaptations for nectar uptake of Prosoecamarinusi that were endemic to the Northern Cape of South Africa. Our study site was a small isolated area of semi-natural habitat, where the long-tubed flowers of Babiana vanzijliae (Iridaceae) were the only nectar source of P. marinusi, and these flies were the only insects with matching proboscis. On average, the proboscis measured 32.63 ± 2.93 mm in length and less than 0.5 mm in diameter. The short labella at the tip are equipped with pseudotracheae that open at the apical margin, indicating that nectar is extracted out of the floral tube with closed labella. To quantify the available nectar resources, measurements of the nectar volume were taken before the flies were active and after observed flower visits. On average, an individual fly took up approximately 1 µL of nectar per flower visit. The measured nectar quantities and the flower geometry allowed estimations of the nectar heights and predictions of necessary proboscis lengths to access nectar in a range of flower tube lengths. [ABSTRACT FROM AUTHOR]