1. Functional expression of a locust tyramine receptor in murine erythroleukaemia cells
- Author
-
J, Poels, M M, Suner, M, Needham, H, Torfs, J, De Rijck, A, De Loof, S J, Dunbar, and J, Vanden Broeck
- Subjects
Mice ,DNA, Complementary ,Base Sequence ,Receptors, Biogenic Amine ,Molecular Sequence Data ,Cyclic AMP ,Tumor Cells, Cultured ,Animals ,Gene Expression ,Calcium ,Grasshoppers ,Leukemia, Erythroblastic, Acute - Abstract
The LCR/MEL system (Locus Control Region/Murine Erythroleukaemia cells) was employed to express and characterize the Locusta migratoria tyramine receptor (TyrLoc), an insect G protein-coupled receptor. Functional agonist-dependent responses were recorded in stable, tyramine receptor expressing cell clones (MEL-TyrLoc). Tyramine elicited a dose-dependent increase of cytosolic Ca2+-ions and an attenuation of forskolin-induced cyclic adenosine monophosphate (AMP) production. Octopamine was shown to be a weak agonist for both responses. In addition, yohimbine proved to be a potent tyramine receptor antagonist. This study reports the first application of the LCR/MEL expression system in functional assays for G protein-coupled receptors and therefore expands the capabilities of this system by exploiting the functionality of the signal transduction pathways.
- Published
- 2002