1. A TRP channel is expressed inSpodoptera littoralisantennae and is potentially involved in insect olfactory transduction
- Author
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Philippe Lucas, Françoise Bozzolan, Bastien Chouquet, Martine Maïbèche-Coisne, Marthe Solvar, Stéphane Debernard, Physiologie de l'Insecte : Signalisation et Communication (PISC), Institut National de la Recherche Agronomique (INRA)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National Agronomique Paris-Grignon (INA P-G), and Université Pierre et Marie Curie - Paris 6 (UPMC)
- Subjects
Male ,Olfactory system ,animal structures ,[SDV]Life Sciences [q-bio] ,media_common.quotation_subject ,Molecular Sequence Data ,TRP CHANNEL ,Insect ,Spodoptera ,OLFACTORY SYSTEM ,CLONING ,ADNC ,03 medical and health sciences ,Transient receptor potential channel ,Transient Receptor Potential Channels ,0302 clinical medicine ,Rapid amplification of cDNA ends ,Complementary DNA ,Botany ,Genetics ,Animals ,EXPRESSION PATTERNS ,Amino Acid Sequence ,Cloning, Molecular ,SPODOPTERA LITTORALIS ,Spodoptera littoralis ,Molecular Biology ,Phylogeny ,030304 developmental biology ,media_common ,Cloning ,0303 health sciences ,biology ,Gene Expression Profiling ,fungi ,Animal Structures ,Gene Expression Regulation, Developmental ,Olfactory Pathways ,Sequence Analysis, DNA ,biology.organism_classification ,3. Good health ,Cell biology ,TRANSDUCTION PATHWAY ,INSECTE ,Insect Science ,sense organs ,030217 neurology & neurosurgery ,Function (biology) ,Signal Transduction - Abstract
International audience; The molecular characterization of post-receptor actors involved in insect olfactory transduction has yet to be understood. We have investigated the presence of a Transient Receptor Potential (TRP) channel in the peripheral olfactory system of the moth Spodoptera littoralis. A cDNA encoding a Lepidopteran TRP channel (TRP gamma) was identified by analysis of a male-antennal EST database and subsequently cloned by RACE PCR. In adult males, the TRP gamma transcript was detected in antennae, at the base of olfactory sensilla. Moreover, TRP gamma was observed in antennae in both pupal and adult stages. This work is the first step in understanding the involvement of TRP gamma in signalling pathways involved in the development and function of the insect olfactory system.
- Published
- 2009
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