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Your search keyword '"Jurat‐Fuentes, J. L."' showing total 2 results
Start Over Author "Jurat‐Fuentes, J. L." Journal insect biochemistry and molecular biology
2 results on '"Jurat‐Fuentes, J. L."'

2. Identification of novel Cry1Ac binding proteins in midgut membranes from Heliothis virescens using proteomic analyses.

Author

Krishnamoorthy M, Jurat-Fuentes JL, McNall RJ, Andacht T, and Adang MJ

Subjects
Animals, Bacillus thuringiensis Toxins, Electrophoresis, Gel, Two-Dimensional, Gastrointestinal Tract chemistry, Gastrointestinal Tract cytology, Glycosylphosphatidylinositols metabolism, Mass Spectrometry, Peptide Mapping, Bacterial Proteins metabolism, Bacterial Toxins metabolism, Endotoxins metabolism, Hemolysin Proteins metabolism, Insect Proteins chemistry, Microvilli chemistry, Moths chemistry, Proteomics, Receptors, Cell Surface chemistry
Abstract

Proteins such as aminopeptidases and alkaline phosphatases, both glycosyl-phosphatidyl-inositol (GPI) anchored proteins, were previously identified as Cry1Ac binding proteins in the Heliothis virescens midgut. To identify additional toxin binding proteins, brush border membrane vesicles from H. virescens larvae were treated with phosphatidyl inositol phospholipase C, and released proteins were resolved by two-dimensional electrophoresis. Protein spots selected by their ability to bind Cry1Ac were identified by MALDI-TOF mass spectrometry coupled to peptide mass fingerprinting (PMF) and database searching. As in previous studies, H. virescens alkaline phosphatase was identified as a Cry1Ac binding protein. V-ATP synthase subunit A and actin were identified as novel Cry1Ac binding proteins in H. virescens. Additional toxin-binding proteins were predicted based on MS/MS fragmentation and de novo sequencing, providing amino acid sequences that were used in database searches to identify a phosphatase and a putative protein of the cadherin superfamily as additional Cry1Ac binding proteins.

Published
2007
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