Search

Your search keyword '"Motohiro Tagaya"' showing total 3 results
Start Over Author "Motohiro Tagaya" Journal inorganic chemistry
3 results on '"Motohiro Tagaya"'

2. Synthesis of Luminescent Nanoporous Silica Spheres Functionalized with Folic Acid for Targeting to Cancer Cells

Author

Toshiyuki Ikoma, Zhefeng Xu, Motohiro Tagaya, and Junzo Tanaka

Subjects
Luminescence, Photoluminescence, chemistry.chemical_element, Inorganic Chemistry, HeLa, Mice, Folic Acid, X-Ray Diffraction, Neoplasms, Specific surface area, Fluorescence microscope, Animals, Humans, Organic chemistry, Physical and Theoretical Chemistry, biology, Chemistry, Nanoporous, Silicon Dioxide, biology.organism_classification, Nanopore, NIH 3T3 Cells, Europium, Porosity, Nanospheres, HeLa Cells, Nuclear chemistry
Abstract

Luminescent europium(III)-doped nanoporous silica nanospheres (Eu:NPS) were successfully synthesized, and a folate N-hydroxysuccinimidyl ester (FA-NHS) molecule as a targeting ligand for cancer cells was immobilized on the nanosphere surfaces through mediation of the 3-aminopropyltriethoxysilane (APTES) adlayer. The ordered nanopores were preserved by the immobilization, and the specific surface area decreased only with the APTES immobilization, suggesting that the FA-NHS was predominantly immobilized on the outer surface of the nanopores. The photoluminescence of the nanospheres functionalized with folic acid (FA) exhibited a characteristic peak due to the interactions (e.g., energy transfer) between FA and Eu(3+), and further the orange luminescence could be clearly detected by fluorescence microscopy in air and water. Furthermore, the nanospheres highly dispersed in cell culture medium exhibited nontoxicity in the cellular proliferation stages of the Hela cancer cells and NIH3T3 fibroblasts and specifically bind to the Hela cells. The nanospheres after the binding and uptake also showed intense luminescence from the outer/inner cell surfaces for the culture time of 4 days. Therefore, the luminescent FA-functionalized Eu:NPS nanospheres could be used for specific targeting and imaging abilities for cancer cells.

Published
2014

3. Synthesis of Luminescent Nanoporous Silica Spheres Functionalized with Folic Acid for Targeting to Cancer Cells.

Author

Motohiro Tagaya, Toshiyuki Ikoma, Zhefeng Xu, and Junzo Tanaka

Subjects
*LUMINESCENT probes, *NANOPOROUS materials, *SILICA, *COMPLEX compounds synthesis, *THERAPEUTIC use of folic acid, *CANCER cells
Abstract

Luminescent europium(III)-doped nanoporous silica nanospheres (Eu:NPS) were successfully synthesized, and a folate N-hydroxysuccinimidyl ester (FA-NHS) molecule as a targeting ligand for cancer cells was immobilized on the nanosphere surfaces through mediation of the 3-aminopropyltriethoxysilane (APTES) adlayer. The ordered nanopores were preserved by the immobilization, and the specific surface area decreased only with the APTES immobilization, suggesting that the FA-NHS was predominantly immobilized on the outer surface of the nanopores. The photoluminescence of the nanospheres functionalized with folic acid (FA) exhibited a characteristic peak due to the interactions (e.g., energy transfer) between FA and Eu3+, and further the orange luminescence could be clearly detected by fluorescence microscopy in air and water. Furthermore, the nanospheres highly dispersed in cell culture medium exhibited nontoxicity in the cellular proliferation stages of the Hela cancer cells and NIH3T3 fibroblasts and specifically bind to the Hela cells. The nanospheres after the binding and uptake also showed intense luminescence from Therefore outer/inner cell surfaces for the culture time of 4 days. Therefore, the luminescent FA-functionalized Eu:NPS nanospheres could be used for specific targeting and imaging abilities for cancer cells. [ABSTRACT FROM AUTHOR]

Published
2014
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results