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Your search keyword '"CAGGIARI, LAURA"' showing total 4 results
Start Over Author "CAGGIARI, LAURA" Journal infectious agents and cancer
4 results on '"CAGGIARI, LAURA"'

2. High serum levels of soluble CD40-L in patients with undifferentiated nasopharyngeal carcinoma: pathogenic and clinical relevance

Author

Caggiari Laura, Guidoboni Massimo, Vaccher Emanuela, Barzan Luigi, Franchin Giovanni, Gloghini Annunziata, Martorelli Debora, Zancai Paola, Bortolin Maria, Mazzucato Mario, Serraino Diego, Carbone Antonino, De Paoli Paolo, and Dolcetti Riccardo

Subjects
Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282, Infectious and parasitic diseases, RC109-216
Abstract

Abstract Background Engagement of CD40 promotes survival of undifferentiated nasopharyngeal carcinoma (UNPC) cells and similar effects are induced by the EBV oncoprotein LMP-1 that is expressed in a fraction of cases. Considering that CD40 may be activated also by the soluble isoform of CD40L (sCD40L), we investigated the serum levels of sCD40L in a series of 61 UNPC patients from Italy, a non-endemic area for this disease. Results At diagnosis, serum samples of UNPC patients contained significantly higher levels of sCD40L than age-matched healthy controls (p < 0.001). High levels of sCD40L (i.e., >18 ng/ml) were more frequently found in patients + lymphoid cells admixed to neoplastic UNPC cells were detected in cases with high serum levels of sCD40L, suggesting that sCD40L is probably produced within the tumor mass. Conclusion sCD40L may contribute to CD40 activation in UNPC cells, particularly of LMP-1-negative cases, further supporting the crucial role of CD40 signalling in the pathogenesis of UNPC. sCD40L levels may be useful to identify UNPC patients with occult distant metastases at presentation.

Published
2007
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4. Protein signature characterizing Helicobacter pylori strains of patients with autoimmune atrophic gastritis, duodenal ulcer and gastric cancer

Author

Ombretta Repetto, Valli De Re, Laura Caggiari, Stefania Zanussi, Mariateresa Casarotto, Renato Cannizzaro, Vincenzo Canzonieri, De Re, Valli, Repetto, Ombretta, Zanussi, Stefania, Casarotto, Mariateresa, Caggiari, Laura, Canzonieri, Vincenzo, and Cannizzaro, Renato

Subjects
0301 basic medicine, Cancer Research, medicine.medical_specialty, Epidemiology, Atrophic gastritis, Comparative proteomic, Adenocarcinoma, Autoimmune atrophic gastritis, Comparative proteomics, DIGE, Duodenal ulcer, Gastric cancer, Helicobacter pylori, lcsh:RC254-282, Gastroenterology, lcsh:Infectious and parasitic diseases, 03 medical and health sciences, chemistry.chemical_compound, Autoimmune atrophic gastriti, Internal medicine, medicine, lcsh:RC109-216, Gel electrophoresis, biology, business.industry, Stomach, Cancer, lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens, medicine.disease, biology.organism_classification, Molecular biology, 030104 developmental biology, Infectious Diseases, medicine.anatomical_structure, Oncology, chemistry, Nucleic acid, business, DNA
Abstract

Background Helicobacter pylori (H. pylori) represents a key factor in the etiology of autoimmune atrophic gastritis (AAG), duodenal ulcer (DU) and gastric cancer (GC). The aim of this study was to characterize the differential protein expression of H. pylori isolated from gastric biopsies of patients affected by either AAG, DU or GC. Methods The H. pylori strains were isolated from endoscopic biopsies from the stomach of patients with gastric disease. Protein profiles of H. pylori were compared by two-dimensional difference in gel electrophoresis (2D-DIGE) coupled with mass spectrometry (MS) for the identification of significantly different spots (Student t-test, p 1.5). These spots corresponded to 35 unique proteins. The identity of 7 protein spots was validated after one-dimensional electrophoresis and MS/MS analyses of excised gel portions. In H. pylori isolated from DU-patients a significant increase in proteins with antioxidant activity emerged (AroQ, AspA, FldA, Icd, OorA and ScoB), together with a higher content of proteins counteracting the high acid environment (KatA and NapA). In H. pylori isolated from AAG-patients proteins neutralizing hydrogen concentrations through organic substance metabolic processes decreased (GroL, TrxB and Tuf). In addition, a reduction of bacterial motility (FlhA) was found to be associated with AAG-H. pylori isolates. In GC-H. pylori strains it was found an increase in nucleic acid-binding proteins (e.g. DnaG, Tuf, RpoA, RplU) which may be involved in a higher demand of DNA- and protein-related processes. Conclusion Our data suggest the presence of specific protein signatures discriminating among H. pylori isolated from either AAG, DU or GC. Changes in protein expression profiles evaluated by DIGE succeeded in deciphering part of the molecular scenarios associated with the different H. pylori-related gastric diseases.

Published
2017

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