Allen, J H, Utley, M, van Den Bosch, H, Nuijten, P, Witvliet, M, McCormick, B A, Krogfelt, K A, Licht, T R, Brown, D, Mauel, M, Leatham, M P, Laux, D C, and Cohen, P S
A minitransposon mutant of Salmonella enterica serovar Typhimurium SR-11, SR-11 Fad(-), is unable to utilize gluconeogenic substrates as carbon sources and is avirulent and immunogenic when administered perorally to BALB/c mice (M. J. Utley et al., FEMS Microbiol. Lett., 163:129-134, 1998). Here, evidence is presented that the mutation in SR-11 Fad(-) that renders the strain avirulent is in the cra gene, which encodes the Cra protein, a regulator of central carbon metabolism.