1. Cell Surface-Associated Elongation Factor Tu Mediates the Attachment of Lactobacillus johnsonii NCC533 (La1) to Human Intestinal Cells and Mucins
- Author
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Martine Rouvet, Dominique Granato, Irene Corthesy-Theulaz, Gabriela Bergonzelli, Laure Marvin, and Raymond David Pridmore
- Subjects
Molecular Sequence Data ,Immunology ,Peptide Elongation Factor Tu ,Microbiology ,Bacterial Adhesion ,Cell Line ,Mice ,HT29 Cells ,Intestinal mucosa ,Cell Wall ,Animals ,Humans ,Amino Acid Sequence ,Interleukin 8 ,Adhesins, Bacterial ,Lactobacillus johnsonii ,Inflammation ,Mice, Inbred BALB C ,Cellular Microbiology: Pathogen-Host Cell Molecular Interactions ,biology ,Interleukin-8 ,Mucin ,Mucins ,biology.organism_classification ,Recombinant Proteins ,In vitro ,Cell biology ,Intestines ,Lactobacillus ,Infectious Diseases ,Biochemistry ,Caco-2 ,Cell culture ,Parasitology ,Caco-2 Cells - Abstract
The aim of this work was to identify Lactobacillus johnsonii NCC533 (La1) surface molecules mediating attachment to intestinal epithelial cells and mucins. Incubation of Caco-2 intestinal epithelial cells with an L. johnsonii La1 cell wall extract led to the recognition of elongation factor Tu (EF-Tu) as a novel La1 adhesin-like factor. The presence of EF-Tu at the surface of La1 was confirmed by analysis of purified outer surface protein extract by immunoblotting experiments, by electron microscopy, and by enzyme-linked immunosorbent assays of live bacteria. Furthermore, tandem mass spectrometry analysis proved that EF-TU was expressed at the La1 surface as an intact molecule. Using recombinant La1 EF-Tu protein, we were able to determine that its binding to intestinal cells and to mucins is pH dependent. Competition experiments suggested that EF-Tu has an important role in La1 mucin binding capacity. In addition, immunomodulation studies performed on HT29 cells showed that EF-Tu recombinant protein can induce a proinflammatory response in the presence of soluble CD14. Our in vitro results indicate that EF-Tu, through its binding to the intestinal mucosa, might participate in gut homeostasis.
- Published
- 2004