1. The Immunodominant Brugia malayi Paramyosin as a Marker of Current Infection with Wuchereria bancrofti Adult Worms
- Author
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Patrick Luquiaud, Steven A. Williams, Sandra Langy, Luc Nicolas, and Catherine Plichart
- Subjects
Adult ,DNA, Complementary ,Recombinant Fusion Proteins ,Molecular Sequence Data ,Immunology ,Antibodies, Helminth ,Tropomyosin ,medicine.disease_cause ,Microbiology ,Microfilaria ,Polynesia ,Diethylcarbamazine ,Brugia malayi ,Immunoscreening ,parasitic diseases ,medicine ,Animals ,Humans ,Wuchereria bancrofti ,Glutathione Transferase ,Antiserum ,Ivermectin ,biology ,Immunodominant Epitopes ,fungi ,Combination chemotherapy ,Helminth Proteins ,biology.organism_classification ,Virology ,Peptide Fragments ,Filariasis ,Filaricides ,Treatment Outcome ,Infectious Diseases ,Antigens, Helminth ,biology.protein ,Parasitology ,Fungal and Parasitic Infections ,Antibody ,Biomarkers ,medicine.drug - Abstract
The full-length cDNA sequence encoding Brugia malayi L3 paramyosin has been isolated by immunoscreening a cDNA library with a mouse antiserum raised against Wuchereria bancrofti L3 infective larvae. A recombinant truncated form of paramyosin was expressed as a glutathione S -transferase fusion protein and used to evaluate humoral responses of adults from a W. bancrofti -endemic area in French Polynesia according to their parasitological status. Immunoglobulin G4 (IgG4) preferentially bound to paramyosin in W. bancrofti -parasitized individuals, in contrast to unparasitized individuals, who harbored neither microfilaria nor Og4C3 adult worm circulating antigen. Reduction of the anti-paramyosin IgG4 titer following combined chemotherapy with diethylcarbamazine and ivermectin was significantly correlated with a reduction in the adult worm burden. This indicates that the presence of paramyosin-reactive IgG4 is associated with the presence of parasites and that reduction can be used as an immunological marker for W. bancrofti clearance.
- Published
- 1998