1. Use of multiplexed tandem PCR to estimate the prevalence and intensity of Theileria orientalis infections in cattle
- Author
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Piyumali K. Perera, Terence W. Spithill, Abdul Jabbar, Hanh T.H. Nguyen, Allan C. Y. Cheng, Grant Rawlin, Aaron R. Jex, J. Malmo, Elizabeth Read, Simon Nyein, and Robin B. Gasser
- Subjects
Microbiology (medical) ,Veterinary medicine ,Future studies ,Genotype ,Severity of Illness Index ,Microbiology ,Asymptomatic ,Theileria ,Multiplex polymerase chain reaction ,Prevalence ,Genetics ,medicine ,Animals ,Asymptomatic Infections ,Molecular Biology ,Ecology, Evolution, Behavior and Systematics ,biology ,Dairy herds ,Outbreak ,biology.organism_classification ,Theileriasis ,Infectious Diseases ,Theileria orientalis ,Immunology ,Cattle ,Female ,medicine.symptom ,Multiplex Polymerase Chain Reaction - Abstract
This study employed a semi-quantitative, multiplexed tandem PCR (MT-PCR) to assess the prevalence and infection intensity of four genotypes (buffeli, chitose, ikeda and type 5) of Theileria orientalis in cattle in Australia. Genomic DNA samples from blood samples (n=448) collected from 27 to 32 dairy cows from each of 15 dairy herds with a history of recent theileriosis outbreaks (Group 1), and from blood samples available from 24 cows with or without oriental theileriosis (Group 2) were tested using MT-PCR. Results revealed that all four genotypes were present in Group 1 cattle; genotype buffeli had the highest prevalence (80.5%), followed by genotypes ikeda (71.4%), chitose (38.6%) and type 5 (20.3%). Genotype ikeda had the highest average infection intensity in the cattle (relating to 55,277 DNA copies), followed by buffeli, chitose and type 5 (6354-51,648 copies). For Group 2, results indicated that genotype ikeda had a significantly higher average intensity of infection than buffeli in symptomatic cattle (P
- Published
- 2015
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