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Diabetic foot ulcers (DFUs) is characterized by a classical triad of neuropathy, ischemia and infection. Patient with Diabetic foot ulcers frequently required lower extremity amputations. Eighty one swab samples were collected from patient with Diabetic foot ulcers, from different hospitals in Basra governorate (Al-Fayhaa General Hospital). All samples were subjected to classical biochemical tests. Gram positive-vitek 2 card system was used for the definitive diagnosis of Gram-positive bacterial samples, 78 samples Gram-positive from 81 samples were diagnosed, and these isolates were found to be of the following species: Staphylococcus aureus, Staphylococcus epidermidis, Staphylococcus xylosus, Staphylococcus haemolyticus, Staphylococcus caprae, Staphylococcus lugdunensis, Staphylococcus warneri, Staphylococcus sciuri, Staphylococcus pseudintermedius, Staphylococcus lentus, Enterococcus faecalis, Streptococcus thoraltensis, Kytococcus sedentarius. Many bacterial species showed multiple-drug resistance, such as Staphylococcus aureus which were more resistant to the following antibiotics: -Oxacillin, Trimethoprim, Tetracycline. The results showed that Staphylococcus aureus, Staphylococcus epidermidis, Staphylococcus xylosus, Staphylococcus haemolyticus, Staphylococcus caprae, Staphylococcus lugdunensis, Staphylococcus warneri, Staphylococcus pseudintermedius, Staphylococcus sciuri, Staphylococcus lentus, Enterococcus faecalis, and Kytococcus sedentarius had the ability to produce biofilm which revealed by various methods, such as Congo red agar qualitative method, and tissue culture plate quantitative method.

The study was carried out to isolate, purify and quantification of bacteriophages against Multiple Drugs Resistance (MDR) P. aeruginosa, and using a bacteriophage as a treatment for mice experimentally. Four groups (Intraperitoneal injection group, local swab group, Positive group, and Negative group) of animals were tested for purpose of using lytic phages of Siphoviridae family as a treatment for MDRs P. aeruginosa isolated from patients suffering from moderate to severe burns wound infection. The current study presents evidence in murine models that animals can be successfully treated with specific bacteriophages that target these MDRs microbes.