8 results on '"Mani M"'
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2. Bacterial identification in the diagnostic laboratory: How much is enough?
- Author
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Kootallur, BN, Thangavelu, CP, and Mani, M
- Published
- 2011
- Full Text
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3. PREVALENCE AND ANTIMICROBIAL SUSCEPTIBILITY PATTERN OF METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS: A MULTICENTRE STUDY
- Author
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Rajaduraipandi, K, Mani, KR, Panneerselvam, K, Mani, M, Bhaskar, M, and Manikandan, P
- Published
- 2006
- Full Text
- View/download PDF
4. PREVALENCE AND ANTIMICROBIAL SUSCEPTIBILITY PATTERN OF METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS:A MULTICENTRE STUDY
- Author
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Rajaduraipandi, K, Mani, KR, Panneerselvam, K, Mani, M, Bhaskar, M, and Manikandan, P
- Abstract
Purpose:Methicillin resistant Staphylococcus aureus(MRSA) is an important nosocomial pathogen. We report the prevalence and antibiotic susceptibility pattern of MRSA in major southern districts of Tamilnadu. Methods:A total of 7172 clinical specimens and 1725 carrier screening samples were collected from different centers and subjected to MRSA screening using conventional microbiological methods. Subsequently the antibiotic sensitivity test was performed for the confirmed MRSA isolates. Results:Out of 906 strains of S. aureus isolated from clinical and carrier samples, 250 (31.1%) and 39 (37.9%) were found to be methicillin resistant respectively. Almost all clinical MRSA strains (99.6%) were resistant to penicillin, 93.6% to ampicillin, and 63.2% towards gentamicin, co-trimoxazole, cephalexin, erythromycin, and cephotaxime. All MRSA strains (100%) of carrier screening samples had resistance to penicillin and about 71.8% and 35.9% were resistant to ampicillin and co-trimoxazole respectively. Multidrug resistance was observed among 63.6% of clinical and 23% of carrier MRSA isolates. However, all strains of clinical and carrier subjects were sensitive to vancomycin. Conclusion:The determination of prevalence and antibiotic sensitivity pattern of MRSA will help the treating clinicians for first line treatment in referral hospitals.
- Published
- 2006
- Full Text
- View/download PDF
5. Seroprevalence of SARS-CoV-2 specific IgG antibodies among eye care workers in South India.
- Author
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Siva Ganesa Karthikeyan R, Rameshkumar G, Gowri Priya C, Lalitha P, Devi R, Iswarya M, and Ravindran RD
- Subjects
- Humans, Immunoglobulin G blood, India epidemiology, Ophthalmology, Optometry, SARS-CoV-2 immunology, Seroepidemiologic Studies, Antibodies, Viral blood, COVID-19 epidemiology, Health Personnel
- Abstract
Purpose: Health care workers are at higher risk of acquiring the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection. This study aims to understand the seroprevalence of anti-SARS-CoV-2 IgG antibody among the eye care workers in South India., Methods: The participants included eye care workers from the nine eye care centres. All the participants were interviewed with a questionnaire to obtain essential information about socio-demographics, past contact with COVID-19 patients and additional information as recommended by Indian Council of Medical Research, India. Serum samples were tested for anti-SARS-CoV-2 IgG antibodies by ELISA., Results: A total of 1313 workers were included and 207 (15.8%) were positive for the SARS-CoV-2 IgG antibody. The seropositivity was higher in the moderate risk group (19.5%) followed by low (18.6%) and high risk (13.7%) groups. The seropositivity was significantly higher among i) day scholars compared to hostellers (OR - 2.22, 1.56 to 3.15, P < 0.0001), ii) individuals with history of flu-like illness (4.57, 3.08-6.78, P < 0.001) or who were symptomatic or in contact with COVID 19 positive cases (2.2, 1.02-4.75, P - 0.043) and iii) individuals with history of systemic illness (2.11, 1.39-3.21, P < 0.001). Individuals (11.97%) who had no history of contact or any illness were also seropositive., Conclusions: The effectiveness of the protective measures taken against COVID infection was evident from the lower percentage of seropositivity in the high risk group. The study highlighted the need to create awareness among individuals to follow strict safety measures even in non-work hours and also in social circles., (Copyright © 2021 Indian Association of Medical Microbiologists. Published by Elsevier B.V. All rights reserved.)
- Published
- 2021
- Full Text
- View/download PDF
6. Hepatitis B virus X protein: The X factor in chronic hepatitis B virus disease progression.
- Author
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Mani M, Vijayaraghavan S, Sarangan G, Barani R, Abraham P, and Srikanth P
- Subjects
- Adult, Alanine Transaminase blood, Cross-Sectional Studies, DNA, Viral genetics, Female, Hepatitis B e Antigens genetics, Hepatitis B e Antigens metabolism, Hepatitis B virus genetics, Hepatitis B virus pathogenicity, Hepatitis B, Chronic blood, Humans, Male, Middle Aged, Phylogeny, Quality Control, Trans-Activators genetics, Viral Regulatory and Accessory Proteins, Hepatitis B, Chronic metabolism, Hepatitis B, Chronic pathology, Trans-Activators metabolism
- Abstract
Introduction: Hepatitis B virus (HBV) is the most common aetiological factor causing hepatocellular carcinoma (HCC). HBx gene plays an enigmatic role in HBV-related HCC. In this study we have analysed amino acid substitutions in HBx from HBV-infected individuals of different clinical stages., Materials and Methods: HBV-infected individuals (n = 93) were recruited in the study. DNA was extracted from plasma, amplified, and DNA sequencing was performed using specific primers targeting HBx gene (540 bp)., Results: Among the study participants, 57% had chronic HBV infection, 30% had chronic liver disease (CLD) and 13% had HBV related HCC. Genotypes such as D1, D2, D3, A1, C2 and B2 were identified of which genotype D2 was predominant (78%). HBxC-terminal deletion was observed in four hepatitis B e antigen (HBeAg) negative participants with CLD. The frequency of aminoacid substitution in proapoptotic domain was higher in HBeAg negative participants including I127V (34%), K130M (34%), V131I (40%). The frequency of double mutation (K130M+V131I) and triple mutation (I127V+K130M+V131I) were found to be higher (32% and 36%) in HBeAg negative participants. Also, we identified L5M substitution (4.3%) in HBeAg positive participants with advanced liver disease., Conclusion: In HBx gene, aminoacid substitutions at positions 127, 130, 131 are associated with poor expression of HBeAg. We suggest screening for HBx aminoacid substitutions especially in patients with HBeAg negative chronic HBV infection to predict the clinical outcome and enable early treatment to prevent disease progression., Competing Interests: None
- Published
- 2019
- Full Text
- View/download PDF
7. Sequencing of Porphyromonas gingivalis from saliva in patients with periodontitis and type 2 diabetes mellitus.
- Author
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Radhakrishnan P, Anbalagan R, Barani R, Mani M, Seshadri KG, and Srikanth P
- Subjects
- Adult, Aged, Bacteroidaceae Infections drug therapy, Bacteroidaceae Infections transmission, Diabetes Mellitus, Type 2 pathology, Doxycycline therapeutic use, Female, Glycated Hemoglobin analysis, Glycemic Index drug effects, Humans, India epidemiology, Male, Middle Aged, Oral Hygiene statistics & numerical data, Periodontitis drug therapy, Periodontitis microbiology, Polymerase Chain Reaction, Porphyromonas gingivalis drug effects, Prospective Studies, RNA, Ribosomal, 16S genetics, Diabetes Complications microbiology, Diabetes Mellitus, Type 2 epidemiology, Periodontitis epidemiology, Porphyromonas gingivalis genetics, Saliva microbiology
- Abstract
Background: Porphyromonas gingivalis is a major periodontal pathogen. Saliva is the most easy, non-invasive microbiological sample for detection of periodontal pathogens., Aim and Objectives: A prospective study on 37 diabetic patients was grouped into well-controlled diabetes with/without periodontitis and uncontrolled diabetic with periodontitis. PCR and sequencing of P. gingivalis was performed in saliva samples., Materials and Methods: DNA was extracted from saliva using Triton X-100 and 16s rRNA gene (404 bp) was amplified by polymerase chain reaction. DNA sequencing was performed for two samples., Results: P. gingivalis was detected in 27.03% (n = 10), of which 30% (n = 9) were diabetic with periodontal disease and 14.3% (n = 1) were diabetic without periodontal disease. The percentage of poor oral hygiene was 50% and 20% in uncontrolled and controlled glycaemic patients, respectively. DNA sequencing of two samples showed 100% identity with the sequences in the GenBank database (Gen Bank accession no: KX640913-KX640914)., Conclusion: Type 2 diabetes mellitus and periodontitis are interlinked. Early detection of P. gingivalis and appropriate treatment with doxycycline will also assist in controlling the glycaemic status., Competing Interests: None
- Published
- 2019
- Full Text
- View/download PDF
8. Nonspecific amplification of human DNA by Streptococcus pneumoniae LytA primer.
- Author
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Thangamony HH, Kumar R, Thangavelu CP, Mariappa M, Mariammal BGV, and Brahmadathan KN
- Subjects
- Base Sequence, Haemophilus influenzae genetics, Humans, Neisseria meningitidis genetics, Polymerase Chain Reaction, Sequence Analysis, DNA, DNA Primers genetics, DNA, Bacterial genetics, Nucleic Acid Amplification Techniques methods, Streptococcus pneumoniae genetics
- Abstract
Background: Determination of various analytical parameters is essential for the validation of primers used for in-house nucleic acid amplification tests. While standardising a high-resolution melt analysis (HRMA) for detection of Streptococcus pneumoniae in acute pyogenic meningitis, we encountered non-specific amplification of certain base pair sequences of human DNA by Centers for Disease Control & Prevention, USA recommended S. pneumoniae LytA primer., Materials and Methods: HRMA was standardised using DNA extracted from an ATCC strain of S. pneumoniae using SP LytA F373 primer and Type-it HRM
TM polymerase chain reaction kit in Rotor-Gene Q Thermal Cycler according to the manufacturer's instructions. Specificity of the primers was determined in dry and wet laboratory experiments against diverse related and unrelated microbial pathogens by HRMA and on DNA extracted from unspiked clinical samples negative for SP DNA. Sensitivity was determined by calculating lower limit of detection threshold in experiments with spiked samples. The amplicon from spiked experiments was sequenced and analysed through Gene Bank., Results: Our dry/wet laboratory experiments showed two separate curves and different Tm values indicating certain non-specific amplification by the primer. Basic Local Alignment Search Tool (BLAST) analysis of the amplicon obtained in the spiked experiment showed sequences of human chromosome 20 associated with Homo sapiens protein tyrosine phosphatase, receptor type T gene. The problem was resolved by stopping the reaction at 30th Ct cycle and observing the Tm values., Conclusion: Since HRMA is done without a specific probe, one should be aware of non-specific amplifications while using primers for HRMA of human clinical samples., Competing Interests: There are no conflicts of interest- Published
- 2018
- Full Text
- View/download PDF
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