1. Contribution of immunofluorescence to identification and characterization of antineutrophil cytoplasmic antibodies in inflammatory bowel disease
- Author
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Nabil Tahri, Hatem Masmoudi, Ali Amouri, S. Haddouk, Youssef Ben Hadj Hmida, and Dorra Bouzid
- Subjects
Adult ,Male ,Pathology ,medicine.medical_specialty ,Enzyme-Linked Immunosorbent Assay ,Immunofluorescence ,Inflammatory bowel disease ,Antibodies, Antineutrophil Cytoplasmic ,Fixatives ,Antigen ,Crohn Disease ,immune system diseases ,medicine ,Humans ,cardiovascular diseases ,skin and connective tissue diseases ,Fluorescent Antibody Technique, Indirect ,Anti-neutrophil cytoplasmic antibody ,Crohn's disease ,biology ,medicine.diagnostic_test ,business.industry ,Gastroenterology ,IIf ,Middle Aged ,medicine.disease ,Ulcerative colitis ,respiratory tract diseases ,Case-Control Studies ,Immunology ,biology.protein ,Colitis, Ulcerative ,Female ,Antibody ,business - Abstract
We evaluated the combined use of different fixatives for the identification of atypical perinuclear anti-neutrophil cytoplasmic antibodies (p-ANCA) in patients with inflammatory bowel diseases (IBD) by indirect immunofluorescence (IIF). Sera from 59 ulcerative colitis (UC) and 37 Crohn’s disease (CD) patients, and from 64 healthy controls were studied. The IIF on ethanol-, formalin-, and methanol-fixed neutrophils was used for the detection of ANCA. Enzyme linked immunosorbant assay (ELISA) was performed to identify the antigens recognized by ANCA. ANCAs were present in 35 of 59 (59.3%) UC patients and in 10 of 37 (27.02%) CD patients. Atypical p-ANCA positivity was strongly associated with UC disease (44.1% in UC vs. 8.1% in CD; p = 0.0002). The combined application of different fixatives contributed to make easy the differentiation between typical p-ANCA and atypical p-ANCA. Atypical p-ANCA determination appears to be a useful parameter for the distinction between UC and CD.
- Published
- 2010