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Background: Diagnosis of Trypanosoma evansi among goat in field is still challenging due to scarce information on clinical manifestation and non availability of rapid reliable diagnostics. Molecular test such as PCR employed to diagnose the T. evansi with accuracy in both blood and tissues. Limited reports are available from different parts of the country on natural infection of T. evansi in goat. Mainly subclinical form of disease occurred in goats owing to low level of parasitaemia of T. evansi. Methods: A Jamunapari goat aged 1.5 years and weighing -25 kg was suffering from circling movement, pale conjunctiva and anorexia and was found positive for T. evansi by buffy coat examination and polymerase chain reaction. Diminazene aceturate was given intramuscularly as 7% water solution at a dose of 3.5 mg/kg b.wt. After 3 days, blood smear and buffy coat were negative for T. evansi and no band was found after running the product on 1.7% agarose gel stained with ethidium bromide. Result: The present study reveals first time report on caprine trypanosomosis from central India by conventional and PCR technique and placed on record. Hence, the goat should also be taken into consideration for control of trypanosomosis as goats are an important source of infection to other animals due to their reservoir nature for T. evansi. [ABSTRACT FROM AUTHOR]

Background: Bovine babesiosis caused by an intraerythrocytic apicomplexan protozoon responsible for the most prevalent and costly tick borne diseases (TBD’s) of cattle throughout the globe. Cerebral babesiosis of bovine is fatal and mainly caused by Babesia bovis. To the knowledge of author, there is no confirm molecular report of Babeisa bigemina caused cerebral babesiosis in cattle. Therefore, authors want to report Babesia bigemina caused cerebral babesiosis on record. Methods: In the year 2015, a Holstein-Friesian cow aged 3 years and weighing approximately 300 kg, was attended at Jabalpur, (M.P.) with the clinical signs of high rise in temperature (104F), recumbency, severe dysponea, peculiar sound during open mouth breathing, pale color of eye conjunctiva and mucous membrane of vagina, convulsions, sever anaemia, paddling of legs at frequent interval. After preparation of peripheral thin blood smear from animal at the site of collection and fixation with methanol, blood sample brought to Department of Veterinary Parasitology, College of Veterinary Science and A.H, Jabalpur and stained by standard protocol for Giemsa staining. Genomic DNA was isolated from the collected blood sample using QIAamp® DNA blood mini kit following the manufacturer’s recommendations and PCR was performed. Conclusion: The thin blood smear examination revealed the presence of Babesia parasite. The species of Babesia was confirmed by molecular amplification of genomic DNA as B. bigemina. This might be the first confirmed report of cerebral babesiosis caused by B. bigemina from Central India. [ABSTRACT FROM AUTHOR]