1. Induction of DNA Damage in Mouse Colorectum by Administration of Colibactin-producing
- Author
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Takumi, Narita, Yuta, Tsunematsu, Noriyuki, Miyoshi, Masami, Komiya, Takahiro, Hamoya, Gen, Fujii, Yuko, Yoshikawa, Michio, Sato, Masanobu, Kawanishi, Haruhiko, Sugimura, Yuji, Iwashita, Yukari, Totsuka, Masaru, Terasaki, Kenji, Watanabe, Keiji, Wakabayashi, and Michihiro, Mutoh
- Subjects
Mice ,Polyketides ,Escherichia coli ,Animals ,Humans ,Female ,Colorectal Neoplasms ,Peptides ,Escherichia coli Infections ,DNA Damage ,Rats ,Research Article - Abstract
Background/Aim: Among colorectal cancer-associated intestinal microbiota, colibactin-producing (clb(+)) bacteria are attracting attention. We aimed to clarify the interaction between clb(+) Escherichia coli and normal colorectal epithelial cells in vivo and in vitro. Materials and Methods: Five-week-old female Balb/c mice were divided in an untreated group, a group treated with clb(+) E. coli isolated from a Japanese patient with colorectal cancer (E. coli-50), and a group treated with non colibactin-producing E. coli (E. coli-50/ΔclbP). Mice were sacrificed at 18 weeks of treatment. Results: Treatment with clb(+) E. coli increased positivity for H2A histone family member X phosphorylated at Ser-139 (γH2AX) in epithelial cells of the luminal surface of the mouse rectum but this did not occur in the E. coli-50/ΔclbP and untreated groups. In an in vitro setting, the ratio of apoptotic cells was increased and cell counts were reduced by treatment with clb(+) E. coli more than in untreated cells and normal rat colorectal epithelial cells. Conclusion: E. coli-50 induced DNA damage in the mouse rectum, possibly by direct interaction between clb(+) E. coli and normal colorectal epithelial cells. Our findings imply that regulation of clb(+) E. coli infection may be a useful strategy for colorectal cancer control.
- Published
- 2021