1. Development of CRISPR_Cas9 genome editing system and its application in rice molecular breeding.
- Author
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Ma, Zengfeng, Wei, Minyi, Zhang, Yuexiong, Qin, Gang, Liu, Chi, Li, Zhenjing, and Huang, Dahui
- Subjects
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GENOME editing , *CRISPRS , *RICE breeding , *GENETIC engineering , *DNA sequencing , *NUCLEIC acids , *NUCLEASES - Abstract
Gene editing is a new genetic engineering technology that uses sequence-specific nucleases to insert, delete, or replace nucleic acid base(s) of the target gene, or introduce exogenous DNA sequences at a specific site in the genome of an organism to achieve the desired modification. Currently, the CRISPR/Cas system is the most widely used genome editing technology because it is relatively simple with high editing efficiency. In this review, we discuss the CRISPR/Cas system's application and its current limitations in rice breeding. Also, we elaborate on possible improved methods regarding its mechanism and development history. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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