1. Transforming growth factor-β1 enhances the secretion of monocyte chemoattractant protein-1 by the IEC-18 intestinal epithelial cell line
- Author
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Jason A. Magida, Dennis W. McGee, and Lisa B. Matagrano
- Subjects
medicine.medical_treatment ,Biology ,digestive system ,Transforming Growth Factor beta1 ,Transforming Growth Factor beta ,parasitic diseases ,medicine ,Animals ,Secretion ,Intestinal Mucosa ,Chemokine CCL2 ,Monocyte ,Chemotaxis ,Cell Biology ,General Medicine ,Epithelium ,Rats ,Cell biology ,Kinetics ,medicine.anatomical_structure ,Cytokine ,Cell culture ,Stem cell ,Interleukin-1 ,Developmental Biology ,Transforming growth factor - Abstract
Intestinal epithelial cells (IEC) are known to produce monocyte chemoattractant protein-1 (MCP-1). However, MCP-1 production, as with many other cytokines, can be regulated by a network of cytokines present in the environment of the IEC. Both IEC and inflammatory cells have been shown to produce transforming growth factor-beta (TGF-beta), and the regulatory effect of this cytokine on MCP-1 secretion by IEC has not been determined. Using the IEC-18 cell line, we have found that TGF-beta1 alone induced the secretion of high levels of MCP-1. Treatment with TGF-beta1 also enhanced the levels of MCP-1 messenger ribonucleic acid. However, costimulation of the cells with TGF-beta1 and interleukin-1beta (IL-1beta) resulted in significant, but less than additive, increases in MCP-1 secretion. Finally, the enhancing effect of TGF-beta1 on MCP-1 secretion was not due to IL-6. These results suggest that TGF-beta1 from IEC or inflammatory cells may significantly enhance the secretion of MCP-1 by IEC and play an important role in inflamed mucosal tissues.
- Published
- 2003
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