1. Human monocyte activation induced by an anti-CD14 monoclonal antibody
- Author
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M. Nausch, Christine Schütt, B. Ringel, A. Plantikow, E. Siegl, Ludwig Jonas, V. Bažil, Friemel H, Peter Neels, Hermann Walzel, and Václav Hořejší
- Subjects
medicine.drug_class ,media_common.quotation_subject ,CD14 ,Immunology ,Lipopolysaccharide Receptors ,Antigens, Differentiation, Myelomonocytic ,In Vitro Techniques ,Monoclonal antibody ,Peripheral blood mononuclear cell ,Monocytes ,chemistry.chemical_compound ,medicine ,Humans ,Immunology and Allergy ,Internalization ,media_common ,biology ,Monocyte ,Zymosan ,Antibodies, Monoclonal ,Molecular biology ,Respiratory burst ,Microscopy, Electron ,medicine.anatomical_structure ,Biochemistry ,chemistry ,Luminescent Measurements ,biology.protein ,Antibody ,Interleukin-1 - Abstract
An anti-CD14 mAb RoMo-1 rapidly induces in human monocytes a transient oxidative burst activity as detected by chemiluminescence assay. Pretreatment of these cells with the mAb markedly suppresses the monocyte chemiluminescence response to opsonized zymosan. In addition, the antibody induces a significant increase of IL-1 production and secretion by mononuclear cells, comparable to a similar effect of rIFN-γ or LPS. Electron microscopy demonstrates internalization of the CD14 molecules after interaction with the mAb in a characteristic receptor-like manner.
- Published
- 1988
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