1. MHC-Restricted Presentation of a Single Repeat of MUC1 Mucin
- Author
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Ramila Philip, Stephen E. Wright, Janet S. Burnside, Melody K Orr, Kenneth E. Dombrowski, and Catherine A. Phillips
- Subjects
biology ,Mucin-1 ,Immunology ,Antigen presentation ,Breast Neoplasms ,chemical and pharmacologic phenomena ,General Medicine ,MHC restriction ,Major histocompatibility complex ,Peripheral blood mononuclear cell ,Peptide Fragments ,Major Histocompatibility Complex ,Epitopes ,CTL ,Variable number tandem repeat ,Tandem Repeat Sequences ,biology.protein ,Cytotoxic T cell ,MUC1 ,T-Lymphocytes, Cytotoxic - Abstract
Major histocompatibility complex (MHC) restriction of antigen presentation of a single mucin1 (MUC1) variable number of tandem repeats peptide (VNTR1) was examined by generating cytotoxic T lymphocytes (CTL) derived from peripheral blood mononuclear cells (PBMC) stimulated with a single repeat MUC1 peptide presented by allogeneic (MHC-independent) or autologous (MHC-dependent) Epstein-Barr Virus (EBV) immortalized B lymphocytes. The ability to generate greater CTL activity against MUC1-expressing tumor cells by stimulation with autologous versus allogeneic EBV-B supports the hypothesis that presentation of a single repeat of MUC1 peptide is MHC-restricted (MHC-dependent).
- Published
- 2007
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