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Your search keyword '"Vanhoutte, L"' showing total 3 results
Start Over Author "Vanhoutte, L" Journal immunity
3 results on '"Vanhoutte, L"'

1. A Subset of Type I Conventional Dendritic Cells Controls Cutaneous Bacterial Infections through VEGFα-Mediated Recruitment of Neutrophils.

Author

Janela B, Patel AA, Lau MC, Goh CC, Msallam R, Kong WT, Fehlings M, Hubert S, Lum J, Simoni Y, Malleret B, Zolezzi F, Chen J, Poidinger M, Satpathy AT, Briseno C, Wohn C, Malissen B, Murphy KM, Maini AA, Vanhoutte L, Guilliams M, Vial E, Hennequin L, Newell E, Ng LG, Musette P, Yona S, Hacini-Rachinel F, and Ginhoux F

Subjects
Acne Vulgaris microbiology, Animals, Antigen Presentation, Chemotaxis, Leukocyte immunology, Dendritic Cells immunology, Ear, External, Gene Expression Regulation, Gene Ontology, Gram-Positive Bacterial Infections microbiology, Humans, Injections, Intradermal, Mice, Mice, Inbred C57BL, Neutrophils metabolism, Propionibacterium acnes, RNA, Messenger biosynthesis, Single-Cell Analysis, Vascular Endothelial Growth Factor A biosynthesis, Vascular Endothelial Growth Factor A genetics, Acne Vulgaris immunology, Dendritic Cells classification, Gram-Positive Bacterial Infections immunology, Neutrophil Infiltration immunology, Vascular Endothelial Growth Factor A immunology
Abstract

Skin conventional dendritic cells (cDCs) exist as two distinct subsets, cDC1s and cDC2s, which maintain the balance of immunity to pathogens and tolerance to self and microbiota. Here, we examined the roles of dermal cDC1s and cDC2s during bacterial infection, notably Propionibacterium acnes (P. acnes). cDC1s, but not cDC2s, regulated the magnitude of the immune response to P. acnes in the murine dermis by controlling neutrophil recruitment to the inflamed site and survival and function therein. Single-cell mRNA sequencing revealed that this regulation relied on secretion of the cytokine vascular endothelial growth factor α (VEGF-α) by a minor subset of activated EpCAM + CD59 + Ly-6D + cDC1s. Neutrophil recruitment by dermal cDC1s was also observed during S. aureus, bacillus Calmette-Guérin (BCG), or E. coli infection, as well as in a model of bacterial insult in human skin. Thus, skin cDC1s are essential regulators of the innate response in cutaneous immunity and have roles beyond classical antigen presentation., (Copyright © 2019 Elsevier Inc. All rights reserved.)

Published
2019
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2. IRF8 Transcription Factor Controls Survival and Function of Terminally Differentiated Conventional and Plasmacytoid Dendritic Cells, Respectively.

Author

Sichien D, Scott CL, Martens L, Vanderkerken M, Van Gassen S, Plantinga M, Joeris T, De Prijck S, Vanhoutte L, Vanheerswynghels M, Van Isterdael G, Toussaint W, Madeira FB, Vergote K, Agace WW, Clausen BE, Hammad H, Dalod M, Saeys Y, Lambrecht BN, and Guilliams M

Subjects
Animals, Interferon Type I metabolism, Mice, Mice, Inbred C57BL, Monocytes metabolism, Monocytes physiology, Promoter Regions, Genetic physiology, T-Lymphocytes metabolism, T-Lymphocytes physiology, Cell Differentiation physiology, Dendritic Cells metabolism, Dendritic Cells physiology, Interferon Regulatory Factors metabolism, Transcription Factors metabolism
Abstract

Interferon regulatory factor-8 (IRF8) has been proposed to be essential for development of monocytes, plasmacytoid dendritic cells (pDCs) and type 1 conventional dendritic cells (cDC1s) and remains highly expressed in differentiated DCs. Transcription factors that are required to maintain the identity of terminally differentiated cells are designated "terminal selectors." Using BM chimeras, conditional Irf8(fl/fl) mice and various promotors to target Cre recombinase to different stages of monocyte and DC development, we have identified IRF8 as a terminal selector of the cDC1 lineage controlling survival. In monocytes, IRF8 was necessary during early but not late development. Complete or late deletion of IRF8 had no effect on pDC development or survival but altered their phenotype and gene-expression profile leading to increased T cell stimulatory function but decreased type 1 interferon production. Thus, IRF8 differentially controls the survival and function of terminally differentiated monocytes, cDC1s, and pDCs., (Copyright © 2016 Elsevier Inc. All rights reserved.)

Published
2016
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3. Conventional and monocyte-derived CD11b(+) dendritic cells initiate and maintain T helper 2 cell-mediated immunity to house dust mite allergen.

Author

Plantinga M, Guilliams M, Vanheerswynghels M, Deswarte K, Branco-Madeira F, Toussaint W, Vanhoutte L, Neyt K, Killeen N, Malissen B, Hammad H, and Lambrecht BN

Subjects
Administration, Inhalation, Adoptive Transfer, Allergens isolation & purification, Animals, Antigens, Dermatophagoides administration & dosage, Antigens, Dermatophagoides isolation & purification, Antigens, Ly genetics, Antigens, Ly immunology, Asthma pathology, CD11b Antigen genetics, CD11b Antigen immunology, Cell Movement, Cell Proliferation, Dendritic Cells transplantation, Gene Expression, Inflammation immunology, Inflammation pathology, Lung immunology, Lung pathology, Lymph Nodes immunology, Lymph Nodes pathology, Mice, Mice, Transgenic, Monocytes immunology, Monocytes transplantation, Natural Cytotoxicity Triggering Receptor 1 genetics, Natural Cytotoxicity Triggering Receptor 1 immunology, Organ Specificity, Receptors, IgG genetics, Receptors, IgG immunology, Allergens immunology, Antigens, Dermatophagoides immunology, Asthma immunology, Dendritic Cells immunology, Immunity, Cellular, Pyroglyphidae immunology, Th2 Cells immunology
Abstract

Dendritic cells (DCs) are crucial for mounting allergic airway inflammation, but it is unclear which subset of DCs performs this task. By using CD64 and MAR-1 staining, we reliably separated CD11b(+) monocyte-derived DCs (moDCs) from conventional DCs (cDCs) and studied antigen uptake, migration, and presentation assays of lung and lymph node (LN) DCs in response to inhaled house dust mite (HDM). Mainly CD11b(+) cDCs but not CD103(+) cDCs induced T helper 2 (Th2) cell immunity in HDM-specific T cells in vitro and asthma in vivo. Studies in Flt3l(-/-) mice, lacking all cDCs, revealed that moDCs were also sufficient to induce Th2 cell-mediated immunity but only when high-dose HDM was given. The main function of moDCs was the production of proinflammatory chemokines and allergen presentation in the lung during challenge. Thus, we have identified migratory CD11b(+) cDCs as the principal subset inducing Th2 cell-mediated immunity in the LN, whereas moDCs orchestrate allergic inflammation in the lung., (Copyright © 2013 Elsevier Inc. All rights reserved.)

Published
2013
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