1. Broadly Neutralizing Anti-HIV Antibody 4E10 Recognizes a Helical Conformation of a Highly Conserved Fusion-Associated Motif in gp41
- Author
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Renate Kunert, Rosa M.F. Cardoso, Ian A. Wilson, Robyn L. Stanfield, James M. Binley, Dennis R. Burton, Michael B. Zwick, and Hermann Katinger
- Subjects
Models, Molecular ,Protein Conformation ,medicine.drug_class ,Amino Acid Motifs ,Molecular Sequence Data ,Static Electricity ,Immunology ,HIV Antibodies ,Crystallography, X-Ray ,Monoclonal antibody ,Epitope ,Cell Line ,Conserved sequence ,Immunoglobulin Fab Fragments ,Protein structure ,Antibody Specificity ,Neutralization Tests ,Cricetinae ,medicine ,Animals ,Immunology and Allergy ,Amino Acid Sequence ,Neutralizing antibody ,Peptide sequence ,Conserved Sequence ,Host cell membrane ,biology ,Hydrogen Bonding ,Viral membrane ,Molecular biology ,HIV Envelope Protein gp41 ,Cell biology ,Infectious Diseases ,biology.protein ,Epitopes, B-Lymphocyte ,Binding Sites, Antibody - Abstract
SummaryBroadly neutralizing monoclonal antibodies to HIV-1 are rare but invaluable for vaccine design. 4E10 is the broadest neutralizing antibody known and recognizes a contiguous and highly conserved epitope in the membrane-proximal region of gp41. The crystal structure of Fab 4E10 was determined at 2.2 Å resolution in complex with a 13-residue peptide containing the gp41 core epitope (NWFDIT). The bound peptide adopts a helical conformation in which the key contact residues, TrpP672, PheP673, IleP675, and ThrP676, map to one face of the helix. The peptide binds in a hydrophobic pocket that may emulate its potential interaction with the host cell membrane. The long CDR H3 of the antibody extends beyond the bound peptide in an orientation that suggests that its apex could contact the viral membrane when 4E10 is bound to its membrane-proximal epitope. These structural insights should assist in the design of immunogens to elicit 4E10-like neutralizing responses.
- Published
- 2005
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