1. Single-cell sequencing of peripheral blood mononuclear cells reveals distinct immune response landscapes of COVID-19 and influenza patients
- Author
-
Xiaoqian Wang, Longqi Liu, Ziyi Li, Fanping Meng, Rui Song, Xiaoyu Wei, Jin Yan, Zhihai Chen, Ziqian Xu, Linnan Zhu, Panyong Mao, Yeya Yu, Beiwei Ye, Guanglin Lei, Shaogeng Zhang, Jian Wang, Haixi Sun, Yang Liu, Yanan Wu, Ning Zhang, Mingyue Wang, Changqing Bai, Jie Zhang, Guanghao Diao, Qumiao Xu, Chuanyu Liu, Yue Yuan, Guizhen Wu, Ling-Xiang Yu, Qiuting Deng, Peipei Liu, Xun Xu, Yingze Zhao, Yunting Huang, Qianqian Gao, Yuhai Bi, George F. Gao, Zhifeng Wang, Penghui Yang, Fu-Sheng Wang, Yi Shi, Guohai Hu, Zhenkun Zhuang, and William J. Liu
- Subjects
0301 basic medicine ,Immunology ,IL-6R ,medicine.disease_cause ,Peripheral blood mononuclear cell ,Article ,Proinflammatory cytokine ,03 medical and health sciences ,0302 clinical medicine ,Immune system ,scRNA-seq ,Influenza A virus ,medicine ,Immunology and Allergy ,STAT3 ,biology ,apoptosis ,Interleukin ,COVID-19 ,030104 developmental biology ,Infectious Diseases ,030220 oncology & carcinogenesis ,biology.protein ,Tumor necrosis factor alpha ,IRF3 ,influenza - Abstract
SUMMARY COVID-19 is a severe infectious disease that is a current global health threat. However, little is known about its hallmarks compared to other infectious diseases. Here, we report the single-cell transcriptional landscape of longitudinally collected peripheral blood mononuclear cells (PBMCs) in both COVID-19 and influenza A virus (IAV)-infected patients. We observed increase of plasma cells in both COVID-19 and IAV patients, and XAF1-, TNF- and FAS-induced T cell apoptosis in COVID-19 patients. Further analyses revealed distinct signaling pathways activated in COVID-19 (STAT1 and IRF3) vs. IAV (STAT3 and NFκB) patients and substantial differences in the expression of key factors. These factors include relatively increase of IL6R and IL6ST expression in COVID-19 patients, but similarly increased IL-6 concentrations compared to IAV patients, supporting the clinical observations of increased pro-inflammatory cytokines in COVID-19 patients. Thus, we provide the landscape of PBMCs and unveil distinct immune response pathways in COVID-19 and IAV patients., Graphical Abstract, Highlights ● We generated a single-cell atlas of PBMCs in both COVID-19 and influenza patients. ● Plasma cells increase significantly in both COVID-19 and influenza patients. ● COVID-19 is featured with XAF1-, TNF- and FAS-induced T cell apoptosis. ● Distinct pathways activate in COVID-19 (STAT1/IRF3) vs. influenza (STAT3/NFκB) patients., COVID-19 and influenza are both respiratory infections with cytokine release syndrome. Zhu et al. use single-cell RNA sequencing of longitudinally collected PBMCs in both patients to reveal distinct immune response landscapes of the two diseases and identify virus-specific cell composition and immune response pathways.
- Published
- 2020