1. Microstructure and Magnetic Properties of LaSrMnO Nanoparticles and Their Application to Cardiac Immunoassay
- Author
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Woo Seung Ham, Jiseok Gim, Ji Sung Lee, Kyu Back Lee, Min Kyung Kim, Jun Hua Wu, and Young Keun Kim
- Subjects
Detection limit ,Materials science ,medicine.diagnostic_test ,biology ,Analytical chemistry ,Nanoparticle ,Crystal structure ,Microstructure ,Fluorescence ,Primary and secondary antibodies ,Electronic, Optical and Magnetic Materials ,Nuclear magnetic resonance ,Immunoassay ,medicine ,biology.protein ,Curie temperature ,Electrical and Electronic Engineering - Abstract
Though enzyme-linked immunosorbent assay is widely used in laboratory medicine, it has limitations due to interactions between enzymes and secondary antibodies. Here, we propose a new cardiac assay scheme for detecting troponin I antigens based on La(1– x )Sr x MnO3 ( $x=0.10$ , 0.15, and 0.25) nanoparticles (NPs). These NPs are synthesized via a modified polyol process followed by heat treatment. An increase in $x$ induces changes in crystal structure and increases both Curie temperature and saturation magnetization. The NPs with $x=0.15$ exhibit a Curie temperature close to the human body temperature. The limit of detection is found to be 0.78 ng/mL. We also confirm that LaSrMnO NPs are reusable after heating the assay plate.
- Published
- 2015
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