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The results of detailed isotopic studies of ice core samples from the Vostok station (East Antarctica) related to the MIS-11 era (the 11th sea isotope stage, i.e. 370–440 thousand years ago) are presented. Reconstruction of paleoclimatic conditions in this period of time was performed using the method of interpretation of the results of isotopic studies of ice, developed by the authors of the article, which is based on the joint analysis of three independent parameters: δD, d-excess, 17 O-excess. The isotopic composition (δD) and the deuterium excess depend on the following three meteorological parameters – the condensation temperature near the Vostok station, relative humidity, and the sea surface temperature at the source of moisture, whereas 17 O-excess depends only on the first two parameters. Accordingly, the proposed method of interpretation allows reconstructing the paleoclimatic conditions (the condensation temperature and surface air temperature at the Vostok station; sea surface temperature and relative humidity above the ocean) in two different regions in past epochs. For the first time, data on minor fluctuations in the relative humidity of the air in themoisture source throughout the MIS-11 era were obtained. The data resulted from the interpretation demonstrated that the relative humidity fluctuated within the measurement error of ±5%. Reconstructed climatic conditions in the era of MIS-11 were compared with published data for stations Vostok and Concordia, aswell as with the marine core data from 94-607 DSDP and ODP 177-1090. The results obtained on the basis of isotopic analysis of ice cores from stations Vostok and Concordia indicated that in the optimum MIS-11the air temperature was4 °C higher, and in the Termination V –8 °C lower than the present-day values. Thesimilarity of data between the marine columns DSDP 94-607 (North Atlantic), ODP 177-1090 (South Ocean)and our results points to the global nature of changes in the sea surface temperature during the MIS-11 era. The coordination of the above results proves the high quality of the methods developed by the authors for measuring and interpreting the isotope composition of ice.

Work on the project focused on the following five areas: 1) field works in Antarctica at Vostok and Concordia stations; 2) experimental and theoretical studies in the field of ice core and paleoclimate research; 3) experimental and theoretical works related to the exploration of subglacial Lake Vostok; 4) development of technology and drilling equipment for deep ice coring and exploration of subglacial lakes; 5) upgrading the analytical instrumentation in the Climate and Environmental Research Laboratory (CERL) of the Arctic and Antarctic Research Institute. The main achievements in the field of ice core and paleoclimate research include 1) further elaboration of a new method of ice core dating, which is based on the link between air content of ice and local insolation, 2) investigation of the possible applications of the 17O-excess measurements in ice core to the paleoclimate research, 3) a better understanding of the mechanisms of the formation of relief-related variations in the isotopic content of an ice core drilled in the area of Antarctic megadunes, and 4) obtaining the first reliable data set on the variations of the 17O-excess in the Vostok core corresponding to marine isotope stage 11. As part of our studies of subglacial Lake Vostok, we have obtained a large body of new experimental data from the new ice core recovered from the 5G-3 borehole to the surface of the subglacial lake. Stacked profiles of isotopic composition, gas content and the size and orientation of the ice crystals in the lake ice have been composed from the data of three replicate cores from boreholes 5G-1, 5G-2 and 5G-3. The study reveals that the concentration of gases in the lake water beneath Vostok is unexpectedly low. A clear signature of the melt water in the surface layer of the lake, which is subject to refreezing on the icy ceiling of Lake Vostok, has been discerned in the three different properties of the accreted ice (the ice texture, the isotopic and gas content of the ice). These sets of data indicate in concert that poor mixing of the melt (and hydrothermal) water with the resident lake water and pronounced spatial and/or temporal variability of local hydrological conditions are likely to be the characteristics of the southern end of the lake. A considerable part of the funding allocated by the RSF to this project was used for upgrading the analytical instrumentation for ice core studies in the CERL of AARI. Using this grant, we purchased and started working with the Picarro L-2140i, a new-generation laser mass analyzer, and set the upgraded mass spectrometer Delta V Plus into operation. The new equipment was used to carry out research planned as part of the project, including the setting up and carrying out of new measurements of 17О in ice cores.

Petrographic studies performed on the continuous basis along the two ice cores obtained from holes 5G-1 and 5G-2 at Vostok Station has allowed to characterize with great details the evolution of the ice texture and fabric in the 232-m thick stratum of accreted ice formed from theLakeVostokwater. Conventionally the whole thickness of accreted ice is divided into two strata: lake ice 1 and lake ice 2. Lake ice 1 (3537–3618 m), formed in the sallow strait50 kmupstream of Vostok, is characterized by presence of disseminated mineral inclusions of Lake Vostok sediments, as well as of «water pockets» that represent frozen water inclusions trapped during the ice accretion. The latter constitute less than 1% of the total ice volume, their mean size is about0.5 cm. Gases trapped by «water pockets» during ice formation transform into crystalline inclusions of mixed gas hydrates. Accretion of lake ice 2 (3618–3769 m) proceeds in the deep part of the lake at a very small rate that does not assume trapping of liquid water inclusions and gases. Both strata of accreted ice are formed by orthotropic crystal growth from pure water. The main tendency in the evolution of accreted ice texture is growth of the mean crystal size with depth as the lake ice becomes younger towards the ice-water interface. The high-amplitude variations of crystal size and orientation observed around this general trend are shown to be linked with temporal and spatial variability of the supercooled melt-water flux from the northern part of the lake towards the ice formation site. The presence of supercooled water at the crystallization front supports persistent preferable growth of ice crystals with sub-horizontally oriented c-axes. The lack of supercooled water in turn support persistent growth of ice crystals with vertical or inclined with respect to the crystallization front c-axis orientation. It means that each of these preferred fabric orientations could serve as an indicator of intensity of melt water flux towards the ice formation site. After completing the isotope measurements on the 5G-2 ice core we plan to use the data on ice texture and fabric obtained in this study together with isotope data for thorough analysis of the peculiarities of water circulation in the southern part ofLakeVostok.

Antarctic subglacial lakes can represent extreme natural habitats for microorganisms from the position of their evolution and adaptation, as well as they can contain the information on Antarctic ice sheet history and climatic changes in their sediments. Now only direct measurements and sampling from these habitats can answer on many fundamental questions. Special precaution should be complied at penetration into these unique relic environments without unfavorable impacts and contamination. A number of recommendations were developed on levels of cleanliness and sterility during direct exploration and research of subglacial environments. Documents considered in the article are the first and necessary steps for appropriate and long-term ecological management of subglacial Antarctic environments. Today there are three projects of subglacial aquatic environment research which are in preparation and realization – the Russian project of Lake Vostok, the similar British project of Lake Ellsworth and the American project on Whillans Ice Stream. The programs of ecological stewardship for direct exploration of these lakes are discussed. All these subglacial aquatic objects of further exploration and research are so various on their structure, age and regime, that only results of all programs as a whole can help to draw us a uniform picture of a subglacial ecological system. Ecological stewardship of these should provide the minimal ecological impact with maximal scientific results. On the basis of existing documents and recommendations the general approaches and the program of ecological stewardship for Lake Vostok research are discussed. Study of drilling fluid, drilling chips, Vostok ice core and the fresh frozen water will allow to make an assessment of biological and chemical contamination as a result of the first penetration and to modify the further stewardship program for the second penetration and direct exploration of lake water.

Between the close-off depth and the bubble-to-hydrate transition zone in polar ice sheets, the geometrical properties of air bubbles, such as number concentration and size of bubbles, are mainly controlled by firn temperature and ice accumulation rate prevailing during the snow to ice transformation [3], and by the bubble compression in the course of bubbly ice densification [13]. This implies that the data on the bubble properties can be used for reconstruction of the past climate change. On the basis of our earlier studies of bubbly ice densification and the new measurements of air bubbles in the Antarctic ice cores, we have developed a theory of bubble evolution in polar ice and propose an inverse procedure for bubble size conversion to specified conditions at the close-off depth. Both outcomes of the research contribute to elaboration of the new paleoclimatological tool based on the bubble properties.