3 results on '"Oliver Domenig"'
Search Results
2. Renin-Angiotensin-Aldosterone System Triple-A Analysis for the Screening of Primary Aldosteronism
- Author
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Paolo Mulatero, Fabrizio Buffolo, Jacopo Burrello, Oliver Domenig, Silvia Monticone, Martina Tetti, Alessio Pecori, and Marko Poglitsch
- Subjects
Adult ,Male ,medicine.medical_specialty ,Secondary hypertension ,Sensitivity and Specificity ,Renin-Angiotensin System ,chemistry.chemical_compound ,Primary aldosteronism ,adrenal glands ,aldosterone ,angiotensin II ,mass spectrometry ,renin-angiotensin system ,Tandem Mass Spectrometry ,Internal medicine ,Renin–angiotensin system ,Hyperaldosteronism ,Internal Medicine ,Medicine ,Humans ,Aldosterone ,business.industry ,Angiotensin II ,Reproducibility of Results ,Middle Aged ,medicine.disease ,Endocrinology ,chemistry ,Female ,Angiotensin I ,Essential Hypertension ,business - Abstract
Primary aldosteronism is recognized as the most frequent cause of secondary hypertension, and its screening is expected to become a routine evaluation in most patients with hypertension. The interference of antihypertensive therapies with the aldosterone-to-renin ratio during screening process is a major confounder. Renin-angiotensin-aldosterone system Triple-A analysis is a novel liquid chromatography/tandem mass spectrometry diagnostic assay that allows simultaneous quantification of aldosterone, equilibrium Ang I (angiotensin I), and Equilibrium Ang II in a single sample of serum. We performed a comparative evaluation of the diagnostic performance of the aldosterone-to-Ang II ratio and 5 renin-based diagnostic ratios, differing in methods to determine aldosterone levels and renin activity in a cohort of 110 patients with hypertension (33 patients with confirmed primary aldosteronism and 77 with essential hypertension). All ratios showed comparable areas under the curves ranging between 0.924 and 0.970 without significant differences between each other. The evaluation of the Ang II-to-Ang I ratio revealed persistent drug intake in some patients as cause for suppressed renin-based diagnostic ratios, while aldosterone-to-Ang II ratio remained unaffected. The Youden index optimal cutoff value for the aldosterone-to-Ang II ratio was 6.6 ([pmol/L]/[pmol/L]) with a sensitivity of 90% and a specificity of 93%, proving noninferiority compared with the aldosterone-to-renin ratio while pointing to the potential for an interference-free application in patients under ACE (angiotensin-converting enzyme) inhibitor therapy. This study shows for the first time the accuracy and reliability of renin-angiotensin-aldosterone system triple-A analysis for the screening of primary aldosteronism that can be applied in clinical routine.
- Published
- 2019
3. Brain Renin-Angiotensin System: Does It Exist?
- Author
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R. Rajkovicova, Oliver Domenig, Frank P.J. Leijten, Ingrid M. Garrelds, Ludovit Paulis, Jeroen Essers, Estrellita Uijl, Bibi S. van Thiel, A.H. Jan Danser, Fatimunnisa Qadri, Luuk te Riet, Marko Poglitsch, Alexandre Goes Martini, Natalia Alenina, Ingrid van der Pluijm, Michael Bader, David Severs, Internal Medicine, Molecular Genetics, Surgery, Clinical Genetics, and Radiotherapy
- Subjects
medicine.medical_specialty ,medicine.drug_class ,Angiotensinogen ,Hippocampus ,Blood Pressure ,030204 cardiovascular system & hematology ,Plasma renin activity ,Renin inhibitor ,Article ,Renin-Angiotensin System ,03 medical and health sciences ,Desoxycorticosterone Acetate ,Mice ,Random Allocation ,0302 clinical medicine ,Fumarates ,Reference Values ,Internal medicine ,Rats, Inbred SHR ,Renin–angiotensin system ,Internal Medicine ,medicine ,Animals ,Mice, Knockout ,Chemistry ,Angiotensin II ,Lisinopril ,Brain ,Amides ,Rats ,Disease Models, Animal ,Endocrinology ,Blood-Brain Barrier ,Knockout mouse ,Hypertension ,Olmesartan ,Perfusion ,030217 neurology & neurosurgery ,medicine.drug - Abstract
Because of the presence of the blood–brain barrier, brain renin–angiotensin system activity should depend on local (pro)renin synthesis. Indeed, an intracellular form of renin has been described in the brain, but whether it displays angiotensin (Ang) I–generating activity (AGA) is unknown. Here, we quantified brain (pro)renin, before and after buffer perfusion of the brain, in wild-type mice, renin knockout mice, deoxycorticosterone acetate salt–treated mice, and Ang II–infused mice. Brain regions were homogenized and incubated with excess angiotensinogen to detect AGA, before and after prorenin activation, using a renin inhibitor to correct for nonrenin-mediated AGA. Renin-dependent AGA was readily detectable in brain regions, the highest AGA being present in brain stem (>thalamus=cerebellum=striatum=midbrain>hippocampus=cortex). Brain AGA increased marginally after prorenin activation, suggesting that brain prorenin is low. Buffer perfusion reduced AGA in all brain areas by >60%. Plasma renin (per mL) was 40× to 800× higher than brain renin (per gram). Renin was undetectable in plasma and brain of renin knockout mice. Deoxycorticosterone acetate salt and Ang II suppressed plasma renin and brain renin in parallel, without upregulating brain prorenin. Finally, Ang I was undetectable in brains of spontaneously hypertensive rats, while their brain/plasma Ang II concentration ratio decreased by 80% after Ang II type 1 receptor blockade. In conclusion, brain renin levels (per gram) correspond with the amount of renin present in 1 to 20 μL of plasma. Brain renin disappears after buffer perfusion and varies in association with plasma renin. This indicates that brain renin represents trapped plasma renin. Brain Ang II represents Ang II taken up from blood rather than locally synthesized Ang II.
- Published
- 2016
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