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Your search keyword '"J. Araujo"' showing total 4 results
Start Over Author "J. Araujo" Journal human immunology
4 results on '"J. Araujo"'

1. Rapid genetic screening for major human leukocyte antigen risk haplotypes in patients with type 1 diabetes from Northeastern Brazil

Author

Luiz Claudio Arraes, Sergio Crovella, José Luiz de Lima Filho, Ludovica Segat, Tarcisio Not, Serena Vatta, Lucas C. Brandao, Rafael Lima Guimarães, and J. Araujo

Subjects
Male, endocrine system, Adolescent, Immunology, Human leukocyte antigen, Polymorphism, Single Nucleotide, law.invention, Young Adult, Gene Frequency, law, Risk Factors, HLA-DQ Antigens, Genetic predisposition, medicine, Immunology and Allergy, Humans, Genetic Predisposition to Disease, Genetic Testing, Child, Genotyping, Polymerase chain reaction, Polymerase, Genetic Association Studies, Genetics, Type 1 diabetes, biology, Histocompatibility Testing, Haplotype, nutritional and metabolic diseases, Infant, General Medicine, medicine.disease, Real-time polymerase chain reaction, Diabetes Mellitus, Type 1, Haplotypes, Child, Preschool, biology.protein, Female, Brazil
Abstract

The aim of this study was to identify in the Brazilian population the frequency of human leukocyte antigen (HLA) DQ2.5 and DQ8 haplotypes conferring risk for type 1 diabetes (T1D), and to validate a new genotyping method aimed at cost reduction and automation. A total of 184 children and adolescents with T1D and 184 healthy individuals from Recife (northeastern Brazil) were analyzed using the conventional polymerase chain reaction–sequence-specific primers HLA genotyping and a newly described Tag-single-nucleotide polymorphism real-time polymerase chain reaction. The Tag-single-nucleotide polymorphism–based HLA genotyping method was successfully validated, proved to be robust, with limited cost and thus could be successfully used for the identification of genetic susceptibility for T1D in areas with limited financial resources. Our findings report for the first time the distribution of DQ2.5 and DQ8 HLA risk haplotypes associated with T1D in northeastern Brazil and evidence a major risk for developing T1D when the heterozygous DQ2.5/DQ8 or the homozygous DQ2.5/DQ2.5 haplotypes are present.

Published
2009

2. Rapid genetic screening for major human leukocyte antigen risk haplotypes in patients with type 1 diabetes from Northeastern Brazil.

Author

Brandao LC, Vatta S, Guimaraes R, Segat L, Araujo J, De Lima Filho JL, Arraes LC, Not T, and Crovella S

Subjects
Adolescent, Brazil, Child, Child, Preschool, Diabetes Mellitus, Type 1 epidemiology, Diabetes Mellitus, Type 1 immunology, Female, Gene Frequency, Genetic Association Studies, Genetic Predisposition to Disease, Haplotypes, Histocompatibility Testing methods, Humans, Infant, Male, Polymorphism, Single Nucleotide, Risk Factors, Young Adult, Diabetes Mellitus, Type 1 genetics, Genetic Testing methods, HLA-DQ Antigens analysis
Abstract

The aim of this study was to identify in the Brazilian population the frequency of human leukocyte antigen (HLA) DQ2.5 and DQ8 haplotypes conferring risk for type 1 diabetes (T1D), and to validate a new genotyping method aimed at cost reduction and automation. A total of 184 children and adolescents with T1D and 184 healthy individuals from Recife (northeastern Brazil) were analyzed using the conventional polymerase chain reaction-sequence-specific primers HLA genotyping and a newly described Tag-single-nucleotide polymorphism real-time polymerase chain reaction. The Tag-single-nucleotide polymorphism-based HLA genotyping method was successfully validated, proved to be robust, with limited cost and thus could be successfully used for the identification of genetic susceptibility for T1D in areas with limited financial resources. Our findings report for the first time the distribution of DQ2.5 and DQ8 HLA risk haplotypes associated with T1D in northeastern Brazil and evidence a major risk for developing T1D when the heterozygous DQ2.5/DQ8 or the homozygous DQ2.5/DQ2.5 haplotypes are present., ((c) 2010 American Society for Histocompatibility and Immunogenetics. Published by Elsevier Inc. All rights reserved.)

Published
2010
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4. Mannose binding lectin gene polymorphisms are associated with type 1 diabetes in Brazilian children and adolescents.

Author

Araujo J, Brandão LA, Guimarães RL, Santos S, Falcão EA, Milanese M, Segat L, Souza PR, de Lima-Filho JL, and Crovella S

Subjects
Adolescent, Age of Onset, Brazil epidemiology, Child, Child, Preschool, Diabetes Mellitus, Type 1 diagnosis, Diabetes Mellitus, Type 1 epidemiology, Female, Gene Frequency, Genotype, Humans, Infant, Male, Diabetes Mellitus, Type 1 genetics, Mannose-Binding Lectin genetics, Polymorphism, Genetic
Abstract

Mannose-binding lectin is an important constituent of the innate immune system, the serum levels of which are greatly affected by polymorphisms of the MBL2 gene: three polymorphisms in exon 1, as well as nucleotide variations in the promoter region of the gene, have been associated with protein deficiency and some infectious and autoimmune disease. The aim of this study was to investigate a possible association between MBL2 gene polymorphisms in patients who have developed type 1 diabetes during childhood and adolescence. We evaluated MBL2 gene polymorphisms in 214 children and adolescents with type 1 diabetes and compared them with a healthy control group, finding significant differences in genotypic and allelic frequencies (p = 0.004 and p = 0.0008, respectively). Our results suggest that patients with type 1 diabetes possessing the 0 allele have a higher risk for developing type 1 diabetes during childhood and adolescence, and that this risk factor is not related to age at diagnosis.

Published
2007
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