1. Stable and Nondisruptive In Vitro/In Vivo Labeling of Mesenchymal Stem Cells by Internalizing Quantum Dots
- Author
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Shinsuke Sakai, Yoshimi Ohyabu, Sunil C. Kaul, Renu Wadhwa, Zeenia Kaul, Kazuki Inoue, Tomokazu Yoshioka, Hajime Mishima, and Toshimasa Uemura
- Subjects
Pathology ,medicine.medical_specialty ,Biocompatibility ,Cell Culture Techniques ,Biology ,Mesenchymal Stem Cell Transplantation ,Chondrocyte ,Osteogenesis ,In vivo ,Quantum Dots ,Genetics ,medicine ,Animals ,Humans ,HSP70 Heat-Shock Proteins ,Molecular Biology ,Adipogenesis ,Staining and Labeling ,Mesenchymal stem cell ,Mesenchymal Stem Cells ,Haplorhini ,Immunohistochemistry ,In vitro ,Rats ,Cell biology ,medicine.anatomical_structure ,Osteocyte ,Chaperone (protein) ,biology.protein ,Molecular Medicine ,Rabbits ,Stem cell ,Chondrogenesis - Abstract
Progress in stem cell research has prioritized the refinement of cell-labeling techniques for in vitro and in vivo basic and therapeutic studies. Although quantum dots, because of their optical properties, are emerging as favorable nanoparticles for bioimaging, substantial refinements or modifications that would improve their biocompatibility are still required. We report here that internalizing quantum dots (i-QDs) generated by their conjugation with an internalizing antibody against a heat shock protein-70 family stress chaperone, mortalin, offered an efficient, genetically noninvasive, nontoxic, and functionally inert way to label mesenchymal stem cells (MSCs). The i-QD-labeled MSCs underwent normal adipocyte, osteocyte, and chondrocyte differentiation in vitro and in vivo, suggesting the potential application of i-QDs in in vivo diagnostics, regenerative and therapeutic medicine.
- Published
- 2009
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