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'Concord' grapevines (Vitis labruscana Bailey) can readily develop iron deficiency-induced leaf chlorosis when grown on calcareous or high pH soils. Iron (Fe) chelates are often applied to the soil to remedy chlorosis but can vary in their stability and effectiveness at high pH. We transplanted own-rooted 1-year-old 'Concord' grapevines into a peat-based medium adjusted to pH 7.5 and fertigated them with 0, 0.5, 1.0, 2.0, or 4 mg.L-1 Fe from Fe-EDDHA [ferric ethylenediamine di (o-hydroxyphenylacetic) acid] to determine the effectiveness of this Fe chelate for alleviating Fe deficiency-induced chlorosis at high pH. Vines were sampled midseason for iron, chlorophyll, CO2 assimilation, and photosystem II quantum efficiency (PSII) and at the end of the season for leaf area, dry weight, and cane length. We found that leaf total Fe concentration was similar across all treatments, but active Fe (extracted with 0.1 N HCI) concentration increased as the rate of Fe-EDDHA increased. Chlorophyll concentration increased curvilinearly as applied Fe increased and was highly correlated with active Fe concentration. CO2 assimilation, stomatal conductance, and PSII were very low without any supplemental Fe and increased rapidly in response to Fe application. Total leaf area, foliar dry weight, and cane length all increased as Fe application increased to 1 mg·L-1 Fe, but above this rate, a further increase in Fe did not significantly increase growth. Our results demonstrate that Fe-EDDHA is very effective in alleviating Fe deficiency-induced leaf chlorosis in 'Concord' grapevines grown at high pH, which provides a foundation for continuing research related to the optimum rate and timing of application of Fe-EDDHA in 'Concord' vineyards on calcareous soils. Compared with total Fe, leaf "active Fe" better indicates the actual Fe status of 'Concord' vines. [ABSTRACT FROM AUTHOR]