1. Box-Behnken Design for Optimizing Ultrasonic-Assisted Enzymatic Extraction of Soluble Dietary Fiber from Pleurotus citrinopilestus
- Author
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Panling Yu, Changxia Yu, Mingjie Chen, Qin Dong, Die Hu, Baosheng Zhang, Mengke Zhang, Jianshuai Ma, Baoting Xu, and Yan Zhao
- Subjects
soluble dietary fiber ,Pleurotus citrinopilestus ,ultrasonic-assisted enzymatic extraction ,response surface methodology ,optimization ,Plant culture ,SB1-1110 - Abstract
Pleurotus citrinopilestus contains a variety of physiologically and pharmacologically active compounds. A key active component among these compounds is dietary fiber, a polysaccharide that exhibits several biological properties. The objective of this study was to assess how soluble dietary fiber (SDF) from Pleurotus citrinopilestus responded to ultrasonic-assisted enzymatic (UAE) extraction. The response surface method (RSM) combined with the Box-Behnken design method (BBD) was used to optimize the yield of SDF. The effects of the liquid-solid ratio (35–55 mL/g), α-amylase concentration (0.5–2.5%), complex protease concentration (0.4–2.0%), and ultrasonication time (15–55 min) on the yield of SDF were examined. The RSM results revealed the optimal liquid-solid ratio (45 mL/g), α-amylase concentration (1.5%), complex protease concentration (1.2%), and ultrasonic time (35 min). The SDF yield was 10.25%, which is close to the predicted value (10.08%).
- Published
- 2023
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