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In the winter season, citrus nursery production faces challenges including shorter days, lower light levels, and lower temperatures that delay vegetative budbreak and scion shoot growth. With the goal of improving the production cycle in the citrus nursery, we investigated the effect of supplemental LED light on the production of bud-grafted citrus trees during short winter days. Three experiments were conducted under different temperature conditions. "Washington" Navel sweet orange (Citrus sinensis) was budded on Carrizo citrange (C. sinensis × Poncirus trifoliata) and Rubidoux trifoliate (P. trifoliata) rootstocks in early December. Light treatments included no supplemental light, day-length extension to 16 h with LED light, and night interruption (1 h of LED light at night). Day-length extension and night interruption were studied with and without preconditioning plants with the respective light treatments for 6 weeks before grafting. Day-length extension increased the scion growth, but only when supplemental heating was provided, implying that low temperatures are a limiting factor for plant growth. Growth effects were stronger when the Navel scion was grafted on Carrizo compared to Rubidoux, likely because of the higher dormancy of the latter rootstock. Night interruption did not affect scion budbreak or growth under any of the tested conditions. Preconditioning enhanced scion growth in some instances. The results suggest that the use of supplemental LED light to extend the day length may increase plant growth during the short winter days, but the effects are limited under low-temperature conditions. [ABSTRACT FROM AUTHOR]

Bacopa monnieri, a cognitive-enhancing herb crucial in health supplements, faces quality variations and contamination by toxic substances in conventional field cultivation, which hinders industrial use. Here, indoor cultivation of diploid (2x) and tetraploid (4x) B. monnieri using hydroponic and soil systems was studied. Soil cultivation promoted longer shoot lengths but resulted in lower biomass and chlorophyll contents compared to hydroponic cultivation. Conversely, soil cultivation significantly elevated total phenolics, total triterpenoids, bacoside A3, and bacopaside X contents in both lines, showing 1.7- to 3.3-fold increases over hydroponic cultivation. Furthermore, 4x plants grown in soil had higher bacopaside II and total bacoside contents than hydroponically grown plants, with 2- and 1.5-fold increases, respectively. Yet, no significant differences were observed in growth and pigment between 2x and 4x lines under the same system. Similarly, no significant differences in bioactive compound productions were found between 2x and 4x hydroponically grown plants. However, in soil, 4x plants exhibited higher total phenolic content, bacopaside II, and total bacoside contents compared to 2x plants. Interestingly, 2x plants grown in soil were the top performers for bacoside production per plant. These findings optimize cultivation practices to meet industry demands, warranting further research into large-scale production techniques. [ABSTRACT FROM AUTHOR]

In the winter season, citrus nursery production faces challenges including shorter days, lower light levels, and lower temperatures that delay vegetative budbreak and scion shoot growth. With the goal of improving the production cycle in the citrus nursery, we investigated the effect of supplemental LED light on the production of bud-grafted citrus trees during short winter days. Three experiments were conducted under different temperature conditions. “Washington” Navel sweet orange (Citrus sinensis) was budded on Carrizo citrange (C. sinensis × Poncirus trifoliata) and Rubidoux trifoliate (P. trifoliata) rootstocks in early December. Light treatments included no supplemental light, day-length extension to 16 h with LED light, and night interruption (1 h of LED light at night). Day-length extension and night interruption were studied with and without preconditioning plants with the respective light treatments for 6 weeks before grafting. Day-length extension increased the scion growth, but only when supplemental heating was provided, implying that low temperatures are a limiting factor for plant growth. Growth effects were stronger when the Navel scion was grafted on Carrizo compared to Rubidoux, likely because of the higher dormancy of the latter rootstock. Night interruption did not affect scion budbreak or growth under any of the tested conditions. Preconditioning enhanced scion growth in some instances. The results suggest that the use of supplemental LED light to extend the day length may increase plant growth during the short winter days, but the effects are limited under low-temperature conditions.

Bacopa monnieri, a cognitive-enhancing herb crucial in health supplements, faces quality variations and contamination by toxic substances in conventional field cultivation, which hinders industrial use. Here, indoor cultivation of diploid (2x) and tetraploid (4x) B. monnieri using hydroponic and soil systems was studied. Soil cultivation promoted longer shoot lengths but resulted in lower biomass and chlorophyll contents compared to hydroponic cultivation. Conversely, soil cultivation significantly elevated total phenolics, total triterpenoids, bacoside A3, and bacopaside X contents in both lines, showing 1.7- to 3.3-fold increases over hydroponic cultivation. Furthermore, 4x plants grown in soil had higher bacopaside II and total bacoside contents than hydroponically grown plants, with 2- and 1.5-fold increases, respectively. Yet, no significant differences were observed in growth and pigment between 2x and 4x lines under the same system. Similarly, no significant differences in bioactive compound productions were found between 2x and 4x hydroponically grown plants. However, in soil, 4x plants exhibited higher total phenolic content, bacopaside II, and total bacoside contents compared to 2x plants. Interestingly, 2x plants grown in soil were the top performers for bacoside production per plant. These findings optimize cultivation practices to meet industry demands, warranting further research into large-scale production techniques.

Nitrate accumulation is a major factor for the secondary salinization of greenhouse soil in China. Our previous study pointed out that a low ratio of red:far-red light (R:FR) can improve salt tolerance in pakchoi under excessive nitrate stress. However, the nitrogen metabolism mechanism is still unclear. To detect the effect of a low R:FR ratio on nitrogen metabolism of pakchoi under excessive nitrate stress, two extra additions of nitrogen of 80 mmol·L−1 NO3− (H80) and 160 mmol·L−1 NO3− (H160) with/without a low R:FR ratio (R:FR = 0.7) were set, and the growth index, chlorophyll content, key enzymes in nitrogen metabolism, nitrate and glutamic acid content and NRT gene expression level of pakchoi leaves were examined. The results indicated that a low ratio of R:FR could alleviate the reduction in growth and chlorophyll content in pakchoi under high-level nitrogen stress (H80 and H160). The activity of nitrate reductase (NR), glutamine synthetase (GS) and glutamine synthetase (GOGAT) decreased under H80 and H160 conditions, except of NR with the H80 treatment. The activity of glutamate dehydrogenase (GDH) increased under H80 treatment, but decreased under H160 treatment. However, the activity of GDH decreased further by reducing the ratio of R:FR. Excessive nitrate stress increased the nitrate content, and a low R:FR ratio could inhibit nitrate accumulation. However, the change in glutamic acid content was significantly increased under a low R:FR ratio without stress. Under the high-nitrogen level treatment (H160), the use of a low ratio of R:FR increased NRT gene expression. Therefore, a low R:FR ratio (R:FR = 0.7) could effectively promote the growth of pakchoi and improve its nitrogen metabolism, thus alleviating the stress effect of a high level of nitrogen in pakchoi. [ABSTRACT FROM AUTHOR]

Tomatoes (Solanum lycopersicum L.) are widely cultivated and consumed, but ripening should be carried out in controlled storage conditions to extend their shelf life and avoid economic losses. The aim of this study was to investigate the effects of visible artificial light on the ripening and quality of fresh market tomatoes stored at a low temperature and high humidity. The postharvest performance with respect to the ripening of organically grown tomatoes in the Toscano cultivar, with a long storage life, was studied in the presence and the absence of visible LED light. The maturation kinetics of the tomatoes was modeled using the Power Law equation. Results showed that tomatoes stored in the presence of light exhibited an increased respiration rate and a faster preclimacteric phase. Lycopene content, total soluble solids, and maturity index increased in the presence of light. Hence, light increased the postharvest ripening of tomatoes, affecting their shelf life. [ABSTRACT FROM AUTHOR]

Microgreens are young, immature vegetables that contain higher concentrations of active compounds compared to mature vegetables and seeds. Radish microgreens are a good source of antioxidants, phenolic compounds, ascorbic acid, carotenoids, and anthocyanins. The production of microgreens is limited by their short shelf life due to higher dark respiration and accelerated senescence. The study was performed on three radish cultivars (Raphanus sativus L.): purple radish (cvP), red radish (cvR), and green radish (cvG). Radish microgreens were grown in chambers with controlled conditions (24 °C and a photoperiod of 16/8 h) under two types of artificial LED light (45 μmol m−2s−1): under white light (B:G:R) and a blue/red light combination (B:2R). The effect of the two types of light was examined on the 3rd, 7th, and 14th day after storage at a low temperature (+4 °C). The physiological status of the three cultivars of radish microgreens was examined by measuring the contents of total soluble phenolics, ascorbic acid, proteins, sugars, dry matter, anthocyanins, carotenoids, and chlorophyll as well as the total antioxidant activity. The results revealed that radish microgreens' antioxidant capacity and phytochemical profile depend on the radish cultivar and on the type of LED light used for cultivation. It was shown that B:2R and red cultivar were most beneficial for the synthesis of most of the determined phytochemicals compared to B:G:R, or the purple and green cultivar, respectively. Storage at a low temperature in darkness slowed down most of the metabolic reactions during the first seven days, thus preserving most of the antioxidant activity. [ABSTRACT FROM AUTHOR]

Nitrate accumulation is a major factor for the secondary salinization of greenhouse soil in China. Our previous study pointed out that a low ratio of red:far-red light (R:FR) can improve salt tolerance in pakchoi under excessive nitrate stress. However, the nitrogen metabolism mechanism is still unclear. To detect the effect of a low R:FR ratio on nitrogen metabolism of pakchoi under excessive nitrate stress, two extra additions of nitrogen of 80 mmol·L−1 NO3− (H80) and 160 mmol·L−1 NO3− (H160) with/without a low R:FR ratio (R:FR = 0.7) were set, and the growth index, chlorophyll content, key enzymes in nitrogen metabolism, nitrate and glutamic acid content and NRT gene expression level of pakchoi leaves were examined. The results indicated that a low ratio of R:FR could alleviate the reduction in growth and chlorophyll content in pakchoi under high-level nitrogen stress (H80 and H160). The activity of nitrate reductase (NR), glutamine synthetase (GS) and glutamine synthetase (GOGAT) decreased under H80 and H160 conditions, except of NR with the H80 treatment. The activity of glutamate dehydrogenase (GDH) increased under H80 treatment, but decreased under H160 treatment. However, the activity of GDH decreased further by reducing the ratio of R:FR. Excessive nitrate stress increased the nitrate content, and a low R:FR ratio could inhibit nitrate accumulation. However, the change in glutamic acid content was significantly increased under a low R:FR ratio without stress. Under the high-nitrogen level treatment (H160), the use of a low ratio of R:FR increased NRT gene expression. Therefore, a low R:FR ratio (R:FR = 0.7) could effectively promote the growth of pakchoi and improve its nitrogen metabolism, thus alleviating the stress effect of a high level of nitrogen in pakchoi.

Microgreens are young, immature vegetables that contain higher concentrations of active compounds compared to mature vegetables and seeds. Radish microgreens are a good source of antioxidants, phenolic compounds, ascorbic acid, carotenoids, and anthocyanins. The production of microgreens is limited by their short shelf life due to higher dark respiration and accelerated senescence. The study was performed on three radish cultivars (Raphanus sativus L.): purple radish (cvP), red radish (cvR), and green radish (cvG). Radish microgreens were grown in chambers with controlled conditions (24 °C and a photoperiod of 16/8 h) under two types of artificial LED light (45 μmol m−2s−1): under white light (B:G:R) and a blue/red light combination (B:2R). The effect of the two types of light was examined on the 3rd, 7th, and 14th day after storage at a low temperature (+4 °C). The physiological status of the three cultivars of radish microgreens was examined by measuring the contents of total soluble phenolics, ascorbic acid, proteins, sugars, dry matter, anthocyanins, carotenoids, and chlorophyll as well as the total antioxidant activity. The results revealed that radish microgreens’ antioxidant capacity and phytochemical profile depend on the radish cultivar and on the type of LED light used for cultivation. It was shown that B:2R and red cultivar were most beneficial for the synthesis of most of the determined phytochemicals compared to B:G:R, or the purple and green cultivar, respectively. Storage at a low temperature in darkness slowed down most of the metabolic reactions during the first seven days, thus preserving most of the antioxidant activity.

Tomatoes (Solanum lycopersicum L.) are widely cultivated and consumed, but ripening should be carried out in controlled storage conditions to extend their shelf life and avoid economic losses. The aim of this study was to investigate the effects of visible artificial light on the ripening and quality of fresh market tomatoes stored at a low temperature and high humidity. The postharvest performance with respect to the ripening of organically grown tomatoes in the Toscano cultivar, with a long storage life, was studied in the presence and the absence of visible LED light. The maturation kinetics of the tomatoes was modeled using the Power Law equation. Results showed that tomatoes stored in the presence of light exhibited an increased respiration rate and a faster preclimacteric phase. Lycopene content, total soluble solids, and maturity index increased in the presence of light. Hence, light increased the postharvest ripening of tomatoes, affecting their shelf life.

Recent improvements in light-emitting diode (LED) technology afford an excellent opportunity to investigate the relationship between different light sources and plant metabolites. Accordingly, the goal of the present study was to determine the effect of different LED (white, blue, and red) treatments on the contents of glucosinolates (glucoiberin, gluconapin, sinigrin, gluconasturtiin, 4-methoxyglucobrassicin, 4-hydroxyglucobrassicin, glucobrassicin, and neoglucobrassicin) and phenolic compounds (4-hydroxybenzonate, catechin, chlorogenic acid, caffeate, gallate, sinapate, and quercetin) in Brassica juncea sprouts. The sprouts were grown in a growth chamber at 25 °C under irradiation with white, blue, or red LED with a flux rate of 90 ^mol m-2 s-1 and a long-day photoperiod (16 h light/8 h dark cycle). Marked differences in desulfoglucosinolate contents were observed in response to treatment with different LEDs and different treatment durations. In addition, the highest total desulfoglucosinolate content was observed in response to white LED light treatment, followed by treatment with red LED light, and then blue LED light. Among the individual desulfoglucosinolates identified in the sprouts, sinigrin exhibited the highest content, which was observed after three weeks of white LED light treatment. The highest total phenolic contents were recorded after one week of white and blue LED light treatment, whereas blue LED irradiation increased the production of most of the phenolic compounds identified, including 4-hydroxybenzonate, gallate, sinapate, caffeate, quercetin, and chlorogenic acid. The production of phenolics decreased gradually with increasing duration of LED light treatment, whereas anthocyanin accumulation showed a progressive increase during the treatment. These findings indicate that white LED light is appropriate for glucosinolate accumulation, whereas blue LED light is effective in increasing the production of phenolic compounds in B. juncea sprouts. [ABSTRACT FROM AUTHOR]