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Assessing donor/recipient HLA compatibility at the eplet level requires second field DNA typings but these are not always available. These can be estimated from lower‐resolution data either manually or with computational tools currently relying, at best, on data containing typing ambiguities. We gathered NGS typing data from 61,393 individuals in 17 French laboratories, for loci A, B, and C (100% of typings), DRB1 and DQB1 (95.5%), DQA1 (39.6%), DRB3/4/5, DPB1, and DPA1 (10.5%). We developed HaploSFHI, a modified iterative maximum likelihood algorithm, to impute second field HLA typings from low‐ or intermediate‐resolution ones. Compared with the reference tools HaploStats, HLA‐EMMA, and HLA‐Upgrade, HaploSFHI provided more accurate predictions across all loci on two French test sets and four European‐independent test sets. Only HaploSFHI could impute DQA1, and solely HaploSFHI and HaploStats provided DRB3/4/5 imputations. The improved performance of HaploSFHI was due to our local and nonambiguous data. We provided explanations for the most common imputation errors and pinpointed the variability of a low number of low‐resolution haplotypes. We thus provided guidance to select individuals for whom sequencing would optimize incompatibility assessment and cost‐effectiveness of HLA typing, considering not only well‐imputed second field typing(s) but also well‐imputed eplets. [ABSTRACT FROM AUTHOR]

HLA‐A*11:463 has one nucleotide change from HLA‐A*11:01:01:01 at nucleotide 508 changing Lysine (146) to Glutamine. [ABSTRACT FROM AUTHOR]

KIR3DP1*00604 differs from KIR3DP1*0060101 by one single nucleotide substitution G > C at position 252. [ABSTRACT FROM AUTHOR]

B*54:47 allele differs from B*54:01:01:01 in codon 74 in exon 2. [ABSTRACT FROM AUTHOR]

HLA‐B*15:270 differs from HLA‐B*15:12:01 by one nucleotide substitution at position 679 in exon 4. [ABSTRACT FROM AUTHOR]

Between January 2018 and June 2021, the Ezer Mizion recruited 223,960 donors. All donors were typed for their HLA class I and II alleles at high resolution by Next Generation Sequencing techniques. Comparison between the sequences obtained from these donors and those in the IPD‐IMGT/HLA Database, revealed 122 Novel HLA alleles that were found in 133 donors. Most of the alleles, 94 (77%) were identified in the HLA class I genes (30, 35, and 29 in HLA‐A, ‐B, and –C, respectively), and 28 (23%) were detected in the HLA class II genes (9 in HLA‐DRB1 and ‐DQB1 and 10 in ‐DPB1). Most of these novel alleles, 106 (86.9%) comprised single nucleotide variation (SNV), 9 (7.4%) present multiple amino acids variation, 4 and 3 were generated because of deletions and insertions, respectively. Ten of these novel alleles were seen to be null alleles. [ABSTRACT FROM AUTHOR]

Characterization of the novel HLA‐A*02:09:01:04 allele in a 36‐year‐old Greek female. [ABSTRACT FROM AUTHOR]

HLA‐DPA1*02:102Q, a novel HLA class II allele detected by next‐generation sequencing. [ABSTRACT FROM AUTHOR]

Allele variants HLA‐C*01:02:01:74Q and ‐C*15:02:01:63 differ from ‐C*01:02:01:01 and ‐15:02:01:01 by a single nucleotide, respectively. [ABSTRACT FROM AUTHOR]

HLA‐B*15:679 and HLA‐C*15:02:01:61 differ from HLA‐B*15:12:01 and HLA‐C*15:02:01:01 by a single nucleotides. [ABSTRACT FROM AUTHOR]

The novel HLA‐B*39:189 allele was characterized using next generation sequencing technology. [ABSTRACT FROM AUTHOR]

Characterization of new alleles, three HLA‐DQA1 and one HLA‐DQB1, bearing non‐synonymous and synonymous mutations, respectively. [ABSTRACT FROM AUTHOR]

HLA‐DQA1*01:04:04 differs from HLA‐DQA1*01:04:01:01 by a single nucleotide substitution at position 225 C > T. [ABSTRACT FROM AUTHOR]

Characterization of six novel HLA alleles by next‐generation sequencing. [ABSTRACT FROM AUTHOR]

The HLA‐DPA1*02:89 allele differs from HLA‐DPA1*02:02:02:01 allele by a single nucleotide in codon 224. [ABSTRACT FROM AUTHOR]

A single nucleotide substitution in exon 5 of HLA‐A*30:01:01:01 results in the novel HLA‐A*30:01:22 allele. [ABSTRACT FROM AUTHOR]

A novel HLA‐B*35 allele, officially designated HLA‐B*35:594, was identified by next‐generation sequencing. [ABSTRACT FROM AUTHOR]

The novel HLA‐DPB1*1449:01N allele differs from HLA‐DPB1*16:01:01:01 by a nucleotide at codon 92 in exon 2. [ABSTRACT FROM AUTHOR]

The novel HLA‐C*14:159 allele was detected during the routine HLA typing for kidney transplantation. [ABSTRACT FROM AUTHOR]

Identification of four new HLA alleles (B*27:265, B*35:569, DRB1*08:117, and DPB1*1435:01) in Brazilian bone marrow donors. [ABSTRACT FROM AUTHOR]

HLA‐B*40:06:01:18 differs from HLA‐B*40:06:01:02 by one nucleotide change in the 5′UTR (T > C). [ABSTRACT FROM AUTHOR]

The novel HLA allele HLA‐A*31:215 differs from HLA‐A*31:01:02:01 by one nucleotide substitution c.G832A in exon 4. [ABSTRACT FROM AUTHOR]

Two different single nucleotide substitutions in intron 1 give rise to the alleles HLA‐DQB1*03:01:01:47 and DQB1*03:01:01:48. [ABSTRACT FROM AUTHOR]

The novel HLA alleles HLA‐A*03:344:02 and ‐DQB1*04:02:24 have synonymous mutations. [ABSTRACT FROM AUTHOR]

The novel HLA‐A*23:122 allele was characterized using next generation sequencing technology. [ABSTRACT FROM AUTHOR]

A synonymous substitution in exon 2 and intronic insertion results in the novel HLA‐DQA1*01:04:07 allele. [ABSTRACT FROM AUTHOR]

We report here the identification by next‐generation sequencing of a novel HLA allele, DRB1*11:308, in a Greek individual as a part of a research project investigating diagnostic and prognostic biomarkers in head and neck cancer (BIOKARETRA). [ABSTRACT FROM AUTHOR]

The novel KIR3DL1*00703 allele differs from the closest allele KIR3DL1*00701 by a single silent mutation. [ABSTRACT FROM AUTHOR]

The novel HLA‐A*01:420N allele has two changes in exon 4 leading to premature stop codon. [ABSTRACT FROM AUTHOR]

A synonymous nucleotide substitution in exon 3 results in the novel HLA‐DQA1*02:01:09:01 allele. [ABSTRACT FROM AUTHOR]

The novel HLA‐C*01:65:02 allele was detected during routine HLA typing. [ABSTRACT FROM AUTHOR]

HLA‐DRB1*14:253 differs from DRB1*14:54:01 by one nucleotide substitution in codon 233 in exon 5. [ABSTRACT FROM AUTHOR]

Compared with the HLA‐C*03:132:01 allele, HLA‐C*03:132:02 shows one nucleotide substitution at position 270A>C. [ABSTRACT FROM AUTHOR]

Identification of the novel HLA‐C*05:279 allele that differs from C*05:01:01:11 by two nucleotide substitutions. [ABSTRACT FROM AUTHOR]

The HLA‐B*53:69 allele differs from HLA‐B*53:01:01:01 by two nucleotide changes in exon 3. [ABSTRACT FROM AUTHOR]

The HLA‐C*12:391 allele has three nucleotide substitutions in exon 3 compared to HLA‐C*12:44 allele. [ABSTRACT FROM AUTHOR]

HLA‐B*57:168 differs from HLA‐B*57:01:01:01 by one nucleotide substitution at codon 325 (TGC > TCC) in exon 7. [ABSTRACT FROM AUTHOR]

Two nucleotide substitutions in exon 4 of HLA‐A*03:01:01:01 results in the novel HLA‐A*03:01:123 allele. [ABSTRACT FROM AUTHOR]

HLA‐DQA1*01:138 is identical to HLA‐DQA1*01:03 except for a single nucleotide substitution in exon 3. [ABSTRACT FROM AUTHOR]

HLA‐A*11:456 has one non‐synonymous nucleotide substitution compared to HLA‐A*11:02:01:01 at position 565 in exon 3. [ABSTRACT FROM AUTHOR]

The novel HLA‐A*03:01:120 allele was characterized using next generation sequencing technology. [ABSTRACT FROM AUTHOR]

HLA‐DRB1*03:201 differs from HLA‐DRB1*03:01 in exon 3 at codon 178 resulting in a proline to serine substitution. [ABSTRACT FROM AUTHOR]

The novel HLA‐DRB1*13:342 allele was characterized using next generation sequencing technology. [ABSTRACT FROM AUTHOR]

The novel HLA‐DPA1*01:130 allele differs from HLA‐DPA1*01:03:01:03 by one nucleotide substitution in Exon 3. [ABSTRACT FROM AUTHOR]

The novel HLA‐DPA1*02:110:02 allele differs from HLA‐DPA1*02:01:01:06 by one nucleotide substitution in exon 4. [ABSTRACT FROM AUTHOR]

The HLA‐C*04:440 allele differs from HLA‐C*04:82:01 by a single non‐synonymous change, c.14C > A. [ABSTRACT FROM AUTHOR]

HLA‐E*01:03:02:25 sequenced from blood and buccal samples of an ALL patient. [ABSTRACT FROM AUTHOR]

HLA‐B*48:58 differs from HLA‐B*48:01:01 by one nucleotide substitution in codon 17 in exon 2. [ABSTRACT FROM AUTHOR]

Three novel HLA‐DRB1 alleles, HLA‐DRB1*07:150, HLA‐DRB1*13:345 and HLA‐DRB1*14:256, were identified. [ABSTRACT FROM AUTHOR]

HLA‐A*24:567N differs from HLA‐A*24:02:01:01 by a single nucleotide deletion at position 149. [ABSTRACT FROM AUTHOR]