13 results on '"Antonio Llombart‐Bosch"'
Search Results
2. Histopathological characterization of small cell osteosarcoma with immunohistochemistry and molecular genetic support. A study of 10 cases
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Franco Bertoni, Michelle Corrigan, Rosa Noguera, Francisco Giner, Isidro Machado, Marco Alberghini, Antonio Pellín, Antonio Llombart-Bosch, and Maureen J. O'Sullivan
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medicine.medical_specialty ,Pathology ,Histology ,Cell ,Cancer ,Anatomical pathology ,General Medicine ,Biology ,medicine.disease ,Small Cell Osteosarcoma ,Pathology and Forensic Medicine ,medicine.anatomical_structure ,medicine ,Immunohistochemistry ,Osteosarcoma ,Histopathology ,Sarcoma - Published
- 2010
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3. Ultrastructural and immunohistochemical characterization of the so-called giant multinucleate cells in cutaneous collagenomas
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Antonio Llombart-Bosch, D Ramón, Carmen Carda, M González-Devesa, David Ramos, and Carlos Monteagudo
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Pathology ,medicine.medical_specialty ,Histology ,Vimentin ,General Medicine ,Anatomy ,Histogenesis ,Biology ,medicine.disease ,Pathology and Forensic Medicine ,Multinucleate ,Giant cell ,Ultrastructure ,Sclerotic fibroma ,medicine ,biology.protein ,Immunohistochemistry ,Fibroma - Abstract
Ultrastructural and immunohistochemical characterization of the so-called giant multinucleate cells in cutaneous collagenomas Aims: This study was undertaken to compare the histopathological, immunohistochemical and ultrastructural features of the so-called giant multinucleatecells in cutaneous collagenomas: giant-cell collagenoma and solitary sclerotic fibroma. Methods and results: We studied four collagenomas: one giant-cell collagenoma and three solitary sclerotic fibromas. All the cases showed an indolent clinical presentation and were histologically constituted by a well-demarcated dome-shaped proliferation of coarse collagen bundles with a varying number of interspersed giant multinucleate cells and stellate mononuclear cells. The immunohistochemical study on paraffin sections revealed that neoplastic cells in both collagenomas were vimentin and CD34-positive, whereas FXIIIa was only expressed in solitary sclerotic fibromas. In regard to the so-called giant multinucleate cells, we have ultrastructurally found that these cells were ‘real’ multinucleate cells in giant-cell collagenoma, whereas in solitary sclerotic fibromas they consisted of closely packed aggregates of individual stellate mononuclear cells. Moreover, perinuclear cisternae focally containing finely textured material of moderate density were unexpectedly found in giant cells of giant-cell collagenoma, a finding which was not observed in solitary sclerotic fibromas. Additionally, a characteristic cell–cell interaction between tumour cells and mast cells was encountered in all collagenomas. Conclusions: This study supports a distinctive immunohistochemical and overall ultrastructural profile of giantmultinucleate cells in giant-cell collagenoma and solitary sclerotic fibroma, which suggests a different pattern of differentiation for these two related cutaneouslesions. more...
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- 2002
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4. Psammomatous malignant melanoma arising in an intradermal naevus
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Antonio Llombart-Bosch, M González-Devesa, Antonio Ferrández, and Carlos Monteagudo
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Pathology ,medicine.medical_specialty ,Histology ,integumentary system ,biology ,Psammoma body ,business.industry ,Melanoma ,Vimentin ,General Medicine ,medicine.disease ,Pathology and Forensic Medicine ,Intradermal Nevus ,medicine ,biology.protein ,Immunohistochemistry ,Differential diagnosis ,business ,Nerve sheath neoplasm ,Immunostaining - Abstract
Psammomatous malignant melanoma arising in an intradermal naevus Aims: A wide variety of differentiation patterns may be found in malignant melanoma. Schwannian features are unusual, and mostly present in the desmoplastic variant. We report the first description of psammoma bodies in malignant melanoma. Methods and results: A malignant melanoma arose in an intradermal naevus of the scalp in a 51-year-old woman, displaying focal neural-like features in the form of rosette-like pseudo-meissnerian alveolar nests, as well as numerous psammoma bodies grouped in a few areas. Tumour cell immunostaining for S100, HMB45, NKI-C3, and vimentin was detected. In addition, both malignant and benign melanocytic cells showed widespread MART-1 immunoreactivity. Differential diagnosis with psammomatous melanotic schwannoma, a feature of Carney’s complex, is particularly emphasized, since dermal variants of this nerve sheath neoplasm have been described. In addition, its potential relationship with cutaneous malignant melanotic neurocristic tumour is discussed. Conclusions: This is, to our knowledge, the first reported case of cutaneous malignant melanoma with psammoma bodies. more...
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- 2001
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5. Cutaneous sclerosing Pacinian-like perineurioma
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Octavio Burgues, I Molina, Antonio Llombart-Bosch, Carlos Monteagudo, Rosa Noguera, and A Revert
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Pathology ,medicine.medical_specialty ,Histology ,medicine.diagnostic_test ,Soft tissue ,General Medicine ,Anatomy ,Biology ,Extraneural ,Perineurial Cell ,Pathology and Forensic Medicine ,medicine.anatomical_structure ,Perineurioma ,medicine ,Immunohistochemistry ,Perineurium ,Chromosome 22 ,Fluorescence in situ hybridization - Abstract
Cutaneous sclerosing Pacinian-like perineurioma Aims: The term perineurioma has been used to designate a variety of clinically and histologically different proliferations of perineurial cells based on immunohistochemical and/or ultrastructural characterization. There are two different groups of neoplasms derived from perineurial cells: extraneural or soft tissue perineuriomas, and intraneural perineuriomas. Recently, a sclerosing variant of cutaneous perineurioma has been described. Methods and results: We report a case of a cutaneous form of perineurioma, combining features of the intraneural and sclerosing varieties, as well as showing a Pacinian pattern of growth. In order to assess the neoplastic nature of the lesion, we performed fluorescence in-situ hybridization (FISH) analysis using a probe which maps to the chromosome band 22q11 and 22q13, allowing us to show deletion or loss of one chromosome 22 in the tumour cells. Conclusions: This case may be considered a new variant of perineurioma with Pacinian-like features, for which we propose the designation ‘sclerosing Pacinian-like perineurioma’. more...
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- 2001
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6. Analysis of cribriform Gleason grade 3 in prostatic carcinoma. A histopathological and immunohistochemical study of 32 cases
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Miguel Martínez-Rodríguez, P Soriano, Antonio Llombart-Bosch, E Mayordomo, and David Ramos
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Male ,Pathology ,medicine.medical_specialty ,Histology ,business.industry ,Prostatic Neoplasms ,General Medicine ,Adenocarcinoma ,Gleason grade ,medicine.disease ,Immunohistochemistry ,Pathology and Forensic Medicine ,Biomarkers, Tumor ,Cribriform ,Carcinoma ,Humans ,Keratins ,Medicine ,business - Published
- 2007
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7. Malignant proliferating trichilemmal tumours: an histopathological and immunohistochemical study of three cases with DNA ploidy and morphometric evaluation
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Ruiz A, Antonio Llombart-Bosch, Carlos Monteagudo, and Juan M. Herrero
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Pathology ,medicine.medical_specialty ,Histology ,biology ,CD34 ,General Medicine ,medicine.disease ,Malignancy ,Pathology and Forensic Medicine ,Proliferating cell nuclear antigen ,Metastasis ,biology.protein ,medicine ,Neoplasm ,Histopathology ,Immunostaining ,Proliferating trichilemmal cyst - Abstract
Aims: Malignant proliferating trichilemmal tumours (MPTT) are rare neoplasms capable of tissue invasion and metastasis, the diagnosis of which is based essentially on histological features. In difficult cases, however, evaluation of additional parameters may be needed to diagnose malignancy. Methods and results We report three cases of MPTT in which, in addition to the histological features, we have determined the DNA ploidy, nuclear area and proliferative fraction. CD34 immunoreactivity has also been tested. Two cases were aneuploid, and one diploid with increased proliferating index. PCNA immunostaining labelled 40% and 80% of tumour cells in aneuploid tumours and 30% of the diploid neoplasm. In all cases, nuclear area was consistent with large pleomorphic tumour cells. No CD34 immunostaining was detected. Conclusions Aneuploidy is common in MPTT, particularly in tumours with a high proliferative fraction. Loss of CD34 immunoreactivity is an additional feature of potential, though limited, value. Therefore, evaluation of the DNA content, proliferation markers and CD34 immunostaining may be helpful in the diagnosis of MPTT. more...
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- 1998
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8. Erythrophagocytic tumour cells in melanoma and squamous cell carcinoma of the skin
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A. Peydró, Antonio Llombart-Bosch, Carlos Monteagudo, C. Illueca, Carmen Carda, and Esperanza Jordá
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Male ,Pathology ,medicine.medical_specialty ,Erythrocytes ,Skin Neoplasms ,Histology ,Malignant histiocytosis ,Biology ,Pathology and Forensic Medicine ,Cytokeratin ,Phagocytosis ,Antigens, Neoplasm ,Biomarkers, Tumor ,medicine ,Humans ,Melanoma ,Histiocyte ,Aged ,Aged, 80 and over ,Mucin-1 ,General Medicine ,medicine.disease ,Immunohistochemistry ,Erythrophagocytosis ,Neoplasm Proteins ,Microscopy, Electron ,Haematopoiesis ,medicine.anatomical_structure ,Epidermoid carcinoma ,Carcinoma, Squamous Cell ,Bone marrow ,Melanoma-Specific Antigens - Abstract
Aims: Erythrophagocytosis is a characteristic feature of tumour cells in malignant histiocytosis, some leukaemias, lymphomas, and also reactive histiocytes in the haemophagocytic syndrome associated with a variety of infections and neoplasms. It has also been found exceptionally in metastatic malignant epithelial cells in bone marrow and lymph nodes. We present two cases, a cutaneous malignant melanoma and an acantholytic squamous cell carcinoma, in which erythrophagocytosis by tumour cells was demonstrable by both light and electron microscopy. Methods and results: The melanocytic and squamous nature of these cells was supported by the immunohistochemical detection of HMB45, S100, and NKI-C3 in the former, and cytokeratin and EMA in the latter, and at ultrastructural level by the presence of melanosomes and tonofilaments, respectively. Conclusions: This is, to our knowledge, the first documented report of erythrophagocytic tumour cells in human melanomas and primary carcinomas. Biological considerations apart, this unusual feature can prove to be of value to avoid a misdiagnosis of a variety of haematopoietic malignancies. more...
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- 1997
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9. Mutational analysis of E-cadherin, β-catenin and APC genes in synovial sarcomas
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Manish Mani, Subramaniam, Silvia, Calabuig-Fariñas, Antonio, Pellin, and Antonio, Llombart-Bosch
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Sarcoma, Synovial ,Genes, APC ,Base Sequence ,DNA Mutational Analysis ,Mutation ,Chromatography, Gel ,Humans ,Cadherins ,beta Catenin - Published
- 2010
10. Dermatofibrosarcoma protuberans: clinical, pathological, and genetic (COL1A1-PDGFB ) study with therapeutic implications
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Carlos Monteagudo, Beatriz Llombart, Onofre Sanmartín, Celia Requena, Juan Luis Vistós, Carlos Serra, Carlos Guillén, Antonio Llombart-Bosch, Sergio Almenar, Andres Poveda, and Jose Antonio López-Guerrero more...
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Adult ,Male ,Pathology ,medicine.medical_specialty ,Histology ,Skin Neoplasms ,Adolescent ,CD34 ,Antineoplastic Agents ,Biology ,Collagen Type I ,Piperazines ,Pathology and Forensic Medicine ,Young Adult ,Dermatofibrosarcoma protuberans ,medicine ,Humans ,Aged ,DNA Primers ,Aged, 80 and over ,PDGFB ,Base Sequence ,Dermatofibrosarcoma ,General Medicine ,Giant-cell fibroblastoma ,Middle Aged ,medicine.disease ,Mohs Surgery ,Collagen Type I, alpha 1 Chain ,Imatinib mesylate ,Pyrimidines ,Fusion transcript ,COL1A1/PDGFB Fusion Gene ,Benzamides ,Imatinib Mesylate ,Female ,Gene Fusion ,Genes, sis - Abstract
Aims: To analyse the presence of collagen type I alpha 1–platelet-derived growth factor beta (COL1A1–PDGFB) transcripts in 20 cases of dermatofibrosarcoma protuberans (DFSP) and to assess the relationship between COL1A1 breakpoints and clinical and histopathological variables. Methods and results: Multiplex reverse transcriptase-polymerase chain reaction was carried out using frozen tissue. Our series contained 14 men and six women. Histologically, most cases were of conventional type (n = 9), followed by fibrosarcoma (n = 4), Bednar tumour (n = 2), sclerosing (n = 2), myoid (n = 1) and atrophic (n = 1) DFSP, and giant cell fibroblastoma (n = 1). Immunohistochemistry revealed CD34 expression in 90% of cases. COL1A1–PDGFB fusion transcripts were present in 89% of cases (exons 18, 19, 20, 25, 26, 31, 33/34, 39, 40, 46, 47 and 48 of COL1A1 with exon 2 of PDGFB). There was no recurrence of DFSP in any of the 19 patients treated by Mohs surgery. A partial response was obtained in the two patients treated with imatinib. Conclusions: The COL1A1–PDGFB fusion was present in all histological subtypes of DFSP, but not all cases expressed the fusion transcript. No association was observed between different COL1A1 breakpoints and clinicopathological parameters. Imatinib mesylate can be useful in locally advanced tumours and metastases. more...
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- 2009
11. Squamoid eccrine ductal carcinoma
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Antonio Llombart-Bosch, Esperanza Jordá, Juan M. Herrero, and Carlos Monteagudo
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Pathology ,medicine.medical_specialty ,Histology ,Eccrine ductal carcinoma ,business.industry ,Immunoenzyme techniques ,Carcinoma ,medicine ,General Medicine ,medicine.disease ,business ,Pathology and Forensic Medicine - Published
- 1998
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12. Clinicopathological and immunohistochemical analysis of 20 cases of Merkel cell carcinoma in search of prognostic markers
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Beatriz Llombart, Antonio Llombart-Bosch, Carmen Carda, José Martín, Jose Antonio López-Guerrero, I Molina, Onofre Sanmartín, Esperanza Jordá, S Almenar, and Carlos Monteagudo
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Male ,Pathology ,Thyroid Nuclear Factor 1 ,Keratin-20 ,Intermediate Filament Proteins ,Lymph node ,Aged, 80 and over ,biology ,Merkel cell carcinoma ,Chromogranin A ,Nuclear Proteins ,General Medicine ,Middle Aged ,Prognosis ,Immunohistochemistry ,DNA-Binding Proteins ,Proto-Oncogene Proteins c-kit ,medicine.anatomical_structure ,Female ,Merkel cell ,medicine.medical_specialty ,Histology ,CD99 ,Synaptophysin ,12E7 Antigen ,Pathology and Forensic Medicine ,Antigens, CD ,Proto-Oncogene Proteins ,Carcinoma ,medicine ,Biomarkers, Tumor ,Chromogranins ,Humans ,Survival analysis ,Aged ,Neoplasm Staging ,Proto-Oncogene Protein c-fli-1 ,Keratin 20 ,medicine.disease ,Survival Analysis ,Carcinoma, Merkel Cell ,Microscopy, Electron ,Ki-67 Antigen ,Multivariate Analysis ,biology.protein ,Trans-Activators ,Neoplasm Recurrence, Local ,Tumor Suppressor Protein p53 ,Cell Adhesion Molecules ,Follow-Up Studies ,Transcription Factors - Abstract
Aims: To evaluate the clinicopathological and immunohistochemical characteristics of Merkel cell carcinoma (MCC) in an attempt to find new, potentially significant, prognostic markers. Methods and results: Clinical data and follow-up, histopathological features (pattern, cell size, thickness, mitoses, vascular invasion, lymphocytic infiltration) and immunohistochemical detection [CK20, thyroid transcription factor (TTF-1), chromogranin A, synaptophysin, p53, Ki67, Fli-1, CD99, c-Kit] were evaluated in 20 cases of MCC. Fli-1 and CD99 were detected in 90% and 55% of cases, respectively. Tumour size > 30 mm, stage II, ‘absent’ lymphocytic infiltration, and the presence of > 50% of Ki67+ tumour cells, were found to be prognostic indicators of disease-free interval (DFI), but only ‘absent’ lymphocytic infiltration constituted an independent prognostic factor of DFI after multivariate analysis. For overall survival, the same variables, together with local recurrence and lymph node involvement, had prognostic significance, with only local recurrence as an independent prognostic factor after multivariate analysis. Conclusions: Absence of lymphocytic infiltration and Ki67 immunoreactivity in more than 50% of tumour cells should be evaluated in conjunction with other well-known prognostic markers in MCC. Furthermore, recognizing that Fli-1 and CD99 expression is commonly found in MCC by immunohistochemistry may avoid misinterpretation in the differential diagnosis of MCC with other small round cell tumours. more...
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- 2005
13. Presence of ganglion cells in Wilm's tumours: a review of the possible neuroepithelial origin of nephroblastoma
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Miguel Cerdá-Nicolás, Antonio Llombart-Bosch, and A. Peydro-Olaya
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Male ,Pathology ,medicine.medical_specialty ,Cytoplasm ,Histology ,Neurofilament ,Neuromuscular Junction ,Biology ,Wilms Tumor ,Pathology and Forensic Medicine ,Lesion ,Neuroblast ,medicine ,Humans ,Child ,Ganglioneuroblastoma ,Cell Nucleus ,Nervous tissue ,Muscles ,Cell Differentiation ,Ganglioneuroma ,General Medicine ,Anatomy ,medicine.disease ,Axons ,Kidney Neoplasms ,Ganglion ,Neuroepithelial cell ,medicine.anatomical_structure ,Neurofibrils ,Ganglia ,medicine.symptom - Abstract
Twenty-seven cases of Wilm's tumour were reviewed in order to confirm the presence of nervous tissue which could imply a ‘neuroepithelial’ origin for these neoplasms. For this purpose a double-silver impregnation technique was used. Groups of ganglion cells with neurofibrils and non-myelinated axon-like processes appeared associated with a fine neurofibrillar network with neuromuscular junctions. Nervous tissue in nephroblastoma is mature and should not be confused with primary ganglioneuroblastoma of the kidney in which neuroblasts in rosette-like groupings are very numerous. A case report of such a lesion is discussed for purposes of comparison. more...
- Published
- 1980
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